60542

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 3072258..3072951 Replicon chromosome
Accession NZ_CP014270
Organism Escherichia coli K-12 strain K-12 DHB4

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag DHB4_RS15265 Protein ID WP_000415584.1
Coordinates 3072655..3072951 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag DHB4_RS15260 Protein ID WP_000650107.1
Coordinates 3072258..3072653 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DHB4_RS15250 3068122..3070380 - 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
DHB4_RS15255 3070518..3072125 - 1608 WP_001295629.1 ABC transporter substrate-binding protein -
DHB4_RS15260 3072258..3072653 - 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
DHB4_RS15265 3072655..3072951 - 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
DHB4_RS15270 3073156..3073638 - 483 WP_000183505.1 transcriptional regulator -
DHB4_RS15275 3073691..3074083 - 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
DHB4_RS15280 3074235..3074894 + 660 WP_001221495.1 two-component system response regulator QseB -
DHB4_RS15285 3074891..3076240 + 1350 WP_000673402.1 two-component system sensor histidine kinase QseC -
DHB4_RS15290 3076286..3076618 - 333 WP_000917684.1 DUF2645 family protein -
DHB4_RS15295 3076937..3077518 + 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
DHB4_RS15300 3077549..3077863 + 315 WP_000958598.1 antibiotic biosynthesis monooxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T60542 WP_000415584.1 NZ_CP014270:c3072951-3072655 [Escherichia coli K-12]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T60542 NZ_CP014270:c3072951-3072655 [Escherichia coli K-12]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT60542 WP_000650107.1 NZ_CP014270:c3072653-3072258 [Escherichia coli K-12]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT60542 NZ_CP014270:c3072653-3072258 [Escherichia coli K-12]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LU65

References