Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 226107..226801 Replicon chromosome
Accession NZ_CP014270
Organism Escherichia coli K-12 strain K-12 DHB4

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag DHB4_RS01085 Protein ID WP_001263489.1
Coordinates 226403..226801 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag DHB4_RS01080 Protein ID WP_000554758.1
Coordinates 226107..226400 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DHB4_RS01060 221739..222236 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
DHB4_RS01065 222460..224172 - 1713 Protein_203 flagellar biosynthesis protein FlhA -
DHB4_RS01070 224144..224929 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
DHB4_RS01075 225000..226055 + 1056 WP_001226164.1 DNA polymerase IV -
DHB4_RS01080 226107..226400 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
DHB4_RS01085 226403..226801 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
DHB4_RS01090 226811..227263 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
DHB4_RS23895 227581..227787 + 207 Protein_209 RtcB family protein -
DHB4_RS01095 227783..228304 + 522 Protein_210 peptide chain release factor H -
DHB4_RS01100 228361..229818 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
DHB4_RS01105 230079..230537 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 231133..231213 + 81 NuclAT_12 - -
- 231133..231213 + 81 NuclAT_12 - -
- 231133..231213 + 81 NuclAT_12 - -
- 231133..231213 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T60512 WP_001263489.1 NZ_CP014270:226403-226801 [Escherichia coli K-12]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T60512 NZ_CP014270:226403-226801 [Escherichia coli K-12]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT60512 WP_000554758.1 NZ_CP014270:226107-226400 [Escherichia coli K-12]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT60512 NZ_CP014270:226107-226400 [Escherichia coli K-12]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References