Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 220063..220604 Replicon chromosome
Accession NZ_CP014270
Organism Escherichia coli K-12 strain K-12 DHB4

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag DHB4_RS01045 Protein ID WP_000615983.1
Coordinates 220063..220341 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag DHB4_RS01050 Protein ID WP_000729703.1
Coordinates 220344..220604 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DHB4_RS01030 217645..218223 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
DHB4_RS01035 218429..219196 + 768 WP_000333380.1 class II glutamine amidotransferase -
DHB4_RS01040 219167..219907 - 741 WP_001225679.1 murein L,D-transpeptidase -
DHB4_RS01045 220063..220341 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
DHB4_RS01050 220344..220604 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
DHB4_RS01055 220814..221563 + 750 WP_000056849.1 C40 family peptidase -
DHB4_RS01060 221739..222236 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
DHB4_RS01065 222460..224172 - 1713 Protein_203 flagellar biosynthesis protein FlhA -
DHB4_RS01070 224144..224929 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T60511 WP_000615983.1 NZ_CP014270:c220341-220063 [Escherichia coli K-12]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T60511 NZ_CP014270:c220341-220063 [Escherichia coli K-12]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT60511 WP_000729703.1 NZ_CP014270:c220604-220344 [Escherichia coli K-12]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT60511 NZ_CP014270:c220604-220344 [Escherichia coli K-12]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References