Detailed information of TA system TA060493

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2213826..2214197
• Replicon: chromosome
• Accession: NZ_CP014269
• Organism: Escherichia coli B strain C3029

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: C3029_RS11210
• Protein ID: WP_001317028.1
• Coordinates: 2214003..2214197 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2213826..2214004 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3029_RS11185	2209781..2211154	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
C3029_RS11190	2211283..2212218	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
C3029_RS23770	2212270..2213505	-	1236	WP_000040858.1	site-specific integrase	-
C3029_RS11200	2213507..2213722	-	216	WP_000079604.1	excisionase XisR	-
C3029_RS11205	2213801..2214010	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	2213826..2214004	+	179	NuclAT_0	-	Antitoxin
-	2213826..2214004	+	179	NuclAT_0	-	Antitoxin
-	2213826..2214004	+	179	NuclAT_0	-	Antitoxin
-	2213826..2214004	+	179	NuclAT_0	-	Antitoxin
C3029_RS11210	2214003..2214197	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
C3029_RS11215	2214254..2215063	-	810	WP_000166319.1	recombination protein RecT	-
C3029_RS11220	2215056..2216155	-	1100	Protein_2138	PD-(D/E)XK nuclease-like domain-containing protein	-
C3029_RS11225	2216257..2216532	-	276	WP_000632297.1	protein RacC	-
C3029_RS11230	2216607..2216777	-	171	WP_001352098.1	conserved protein, Rac prophage	-
C3029_RS11235	2216777..2216998	-	222	WP_000560223.1	killing protein KilR	-
C3029_RS11240	2217440..2217928	+	489	WP_001312793.1	superinfection exclusion protein B	-
C3029_RS11245	2217925..2218080	-	156	WP_001169151.1	YdaF family protein	-
C3029_RS11250	2218224..2218436	+	213	WP_012775985.1	phage lysis protein EssD	-
C3029_RS11255	2218441..2218785	+	345	WP_000193293.1	YdfR family protein	-
C3029_RS11260	2218751..2219023	-	273	WP_000370546.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2205011..2224335	19324

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T60493 WP_001317028.1 NZ_CP014269:c2214197-2214003 [Escherichia coli B]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T60493 NZ_CP014269:c2214197-2214003 [Escherichia coli B]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT60493 NZ_CP014269:2213826-2214004 [Escherichia coli B]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin