Detailed information of TA system TA060488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1014119..1014813
• Replicon: chromosome
• Accession: NZ_CP014269
• Organism: Escherichia coli B strain C3029

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: C3029_RS04945
• Protein ID: WP_001263493.1
• Coordinates: 1014415..1014813 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: C3029_RS04940
• Protein ID: WP_000554757.1
• Coordinates: 1014119..1014412 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3029_RS04920	1009759..1010256	+	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-
C3029_RS04925	1010472..1012184	-	1713	Protein_914	flagellar biosynthesis protein FlhA	-
C3029_RS04930	1012156..1012941	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
C3029_RS04935	1013012..1014067	+	1056	WP_001226164.1	DNA polymerase IV	-
C3029_RS04940	1014119..1014412	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C3029_RS04945	1014415..1014813	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C3029_RS04950	1014823..1015275	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
C3029_RS04955	1015521..1015724	+	204	Protein_920	RNA ligase RtcB family protein	-
C3029_RS04960	1015723..1016244	+	522	Protein_921	peptide chain release factor H	-
C3029_RS04965	1016301..1017758	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
C3029_RS04970	1018019..1018477	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
C3029_RS04975	1018569..1019813	+	1245	WP_000189541.1	esterase FrsA	-
-	1019073..1019153	+	81	NuclAT_13	-	-
-	1019073..1019153	+	81	NuclAT_13	-	-
-	1019073..1019153	+	81	NuclAT_13	-	-
-	1019073..1019153	+	81	NuclAT_13	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	990442..1021095	30653

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T60488 WP_001263493.1 NZ_CP014269:1014415-1014813 [Escherichia coli B]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T60488 NZ_CP014269:1014415-1014813 [Escherichia coli B]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT60488 WP_000554757.1 NZ_CP014269:1014119-1014412 [Escherichia coli B]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT60488 NZ_CP014269:1014119-1014412 [Escherichia coli B]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6