60461

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1006749..1007290 Replicon chromosome
Accession NZ_CP014268
Organism Escherichia coli B strain C2566

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag C2566_RS04890 Protein ID WP_000615981.1
Coordinates 1006749..1007027 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C2566_RS04895 Protein ID WP_000729704.1
Coordinates 1007030..1007290 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2566_RS04875 1004331..1004909 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
C2566_RS04880 1005115..1005882 + 768 WP_000333379.1 class II glutamine amidotransferase -
C2566_RS04885 1005853..1006593 - 741 WP_001225679.1 murein L,D-transpeptidase -
C2566_RS04890 1006749..1007027 - 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C2566_RS04895 1007030..1007290 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C2566_RS04900 1007476..1008249 + 774 WP_001353765.1 C40 family peptidase -
C2566_RS04905 1008425..1008922 + 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
C2566_RS04910 1009138..1010850 - 1713 Protein_914 flagellar biosynthesis protein FlhA -
C2566_RS04915 1010822..1011607 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 989108..1019761 30653


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T60461 WP_000615981.1 NZ_CP014268:c1007027-1006749 [Escherichia coli B]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T60461 NZ_CP014268:c1007027-1006749 [Escherichia coli B]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT60461 WP_000729704.1 NZ_CP014268:c1007290-1007030 [Escherichia coli B]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT60461 NZ_CP014268:c1007290-1007030 [Escherichia coli B]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6Z6

References