Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 26529..27121 Replicon plasmid unnamed3
Accession NZ_CP014236
Organism Moraxella osloensis strain CCUG 350

Toxin (Protein)


Gene name graT Uniprot ID A0A120KR99
Locus tag AXE82_RS11320 Protein ID WP_062335123.1
Coordinates 26840..27121 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A120KRC1
Locus tag AXE82_RS11315 Protein ID WP_062335121.1
Coordinates 26529..26825 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXE82_RS11295 22158..23129 + 972 WP_062333364.1 IS481 family transposase -
AXE82_RS11300 23647..25032 + 1386 WP_062335116.1 replication initiation protein -
AXE82_RS11305 25111..25872 + 762 WP_062335118.1 ParA family protein -
AXE82_RS11310 25869..26204 + 336 WP_062335120.1 hypothetical protein -
AXE82_RS11315 26529..26825 - 297 WP_062335121.1 HigA family addiction module antidote protein Antitoxin
AXE82_RS11320 26840..27121 - 282 WP_062335123.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AXE82_RS11325 27367..27972 + 606 WP_062335159.1 recombinase family protein -
AXE82_RS11330 28079..28375 + 297 WP_062335125.1 BrnT family toxin -
AXE82_RS11335 28347..28661 + 315 WP_062335126.1 BrnA antitoxin family protein -
AXE82_RS11340 28943..29353 + 411 WP_065252196.1 transposase -
AXE82_RS11345 29350..29721 + 372 WP_062330255.1 IS66 family insertion sequence element accessory protein TnpB -
AXE82_RS11350 29806..31551 + 1746 WP_082741375.1 IS66 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..57463 57463
- flank IS/Tn - - 22158..23129 971


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(11-63)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10798.23 Da        Isoelectric Point: 6.8610

>T60442 WP_062335123.1 NZ_CP014236:c27121-26840 [Moraxella osloensis]
MIVNFACDKTKTLFTTGNTRHFSSIKKVAERKLLQLDNAITLEDLRSPPGNHLEALFGDREGQHSIRINDQWRICFVWTQ
EGPTEVEIVDYHR

Download         Length: 282 bp

>T60442 NZ_CP014236:c27121-26840 [Moraxella osloensis]
ATGATTGTTAATTTTGCATGTGATAAAACTAAAACACTTTTTACAACAGGCAACACACGTCATTTCTCTTCAATTAAAAA
AGTTGCTGAAAGAAAATTATTGCAATTAGATAATGCGATTACTTTAGAAGACTTGCGTTCTCCGCCAGGTAATCACCTTG
AAGCCCTATTTGGCGACAGAGAAGGTCAGCACAGTATTCGAATCAATGATCAATGGCGGATCTGCTTTGTATGGACACAA
GAAGGTCCGACAGAAGTAGAAATTGTAGATTACCATCGATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11046.78 Da        Isoelectric Point: 7.2020

>AT60442 WP_062335121.1 NZ_CP014236:c26825-26529 [Moraxella osloensis]
MKNGMRPIHPGEILREDYLAPLEMSANALAKAIGVTPARINEIVREERGITADTALRLAKYFGGNAKFWLNLQTTYELRL
AETQDTHFLDKIMPLAFS

Download         Length: 297 bp

>AT60442 NZ_CP014236:c26825-26529 [Moraxella osloensis]
ATGAAAAATGGTATGAGACCTATTCATCCAGGTGAAATTTTGCGAGAAGACTATCTTGCACCGCTAGAGATGAGCGCCAA
TGCCTTAGCGAAGGCTATCGGCGTCACACCTGCCCGTATTAATGAAATCGTGCGCGAGGAACGTGGTATTACTGCTGATA
CGGCACTACGCCTTGCTAAATATTTTGGTGGTAATGCTAAGTTTTGGCTAAACTTACAAACCACTTACGAGCTACGACTA
GCTGAAACCCAAGATACGCATTTTTTAGATAAGATTATGCCATTAGCTTTCAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A120KR99

Antitoxin

Source ID Structure
AlphaFold DB A0A120KRC1

References