Detailed information of TA system TA060436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1712733..1713312
• Replicon: chromosome
• Accession: NZ_CP014232
• Organism: Actinomyces oris strain T14V

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0X8K296
• Locus tag: AXE84_RS06935
• Protein ID: WP_010613968.1
• Coordinates: 1712733..1713014 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AXE84_RS06940
• Protein ID: WP_060957350.1
• Coordinates: 1713001..1713312 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXE84_RS06925	1709199..1711265	+	2067	WP_060957348.1	hypothetical protein	-
AXE84_RS06930	1711303..1712373	-	1071	WP_060957349.1	alpha/beta hydrolase	-
AXE84_RS06935	1712733..1713014	+	282	WP_010613968.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXE84_RS06940	1713001..1713312	+	312	WP_060957350.1	HigA family addiction module antidote protein	Antitoxin
AXE84_RS06945	1713350..1714804	+	1455	WP_060957351.1	NAD(P)/FAD-dependent oxidoreductase	-
AXE84_RS06950	1714912..1715361	+	450	WP_060957352.1	RpiB/LacA/LacB family sugar-phosphate isomerase	-
AXE84_RS06955	1715530..1716354	+	825	WP_060957353.1	class I SAM-dependent methyltransferase	-
AXE84_RS06960	1716409..1716858	+	450	WP_060957354.1	RpiB/LacA/LacB family sugar-phosphate isomerase	-
AXE84_RS06965	1717239..1717979	+	741	WP_060957355.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (9-88)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10800.23 Da
• Isoelectric Point: 8.0772

>T60436 WP_010613968.1 NZ_CP014232:1712733-1713014 [Actinomyces oris]
VIRSFGSKDTERLWHRERVPSIDPRIQRVALRELRQIASVQSVDELRVPPGNRLEVLKGDRAGQHSIRVNDQWRICFVWT
GAGAEEVEIVDYH

Nucleotide

• Length: 282 bp

>T60436 NZ_CP014232:1712733-1713014 [Actinomyces oris]
GTGATCAGGTCGTTCGGTAGTAAGGACACTGAGCGGTTGTGGCACCGAGAACGAGTCCCATCGATTGACCCTCGCATACA
ACGGGTTGCCTTGCGTGAGTTACGTCAGATCGCTTCTGTGCAGTCTGTCGATGAGCTGAGAGTGCCGCCGGGGAACCGAC
TCGAGGTGCTCAAAGGTGATCGGGCCGGCCAGCACAGCATCAGAGTGAATGACCAGTGGCGGATCTGCTTCGTATGGACT
GGCGCTGGAGCGGAGGAGGTTGAGATCGTTGACTACCACTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11406.06 Da
• Isoelectric Point: 6.2164

>AT60436 WP_060957350.1 NZ_CP014232:1713001-1713312 [Actinomyces oris]
MTTTETNSKHAPVHPGEVLMEDFIEGFGITQNKLAVAIGVPPRRINEIVHGKRAVTADTALRLGKYFGVSAQFWLNLQTQ
YDLDVAEDRLSMQIDAITPLRVA

Nucleotide

• Length: 312 bp

>AT60436 NZ_CP014232:1713001-1713312 [Actinomyces oris]
TTGACTACCACTGAAACGAACAGCAAGCACGCCCCTGTTCATCCGGGGGAGGTCCTCATGGAGGACTTCATCGAGGGATT
CGGTATCACCCAGAACAAACTGGCTGTTGCTATCGGAGTGCCGCCGAGGCGTATCAATGAGATCGTGCACGGTAAGCGTG
CGGTGACTGCGGATACGGCGCTGCGGCTCGGCAAGTACTTCGGGGTATCAGCGCAGTTCTGGTTGAACCTGCAGACCCAG
TATGACCTTGACGTGGCGGAGGACCGTCTTTCGATGCAGATCGATGCCATCACTCCTCTGCGAGTGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0X8K296