Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3656874..3657483 Replicon chromosome
Accession NZ_CP014226
Organism Halomonas chromatireducens strain AGD 8-3

Toxin (Protein)


Gene name higB Uniprot ID A0A0X8HH20
Locus tag LOKO_RS16910 Protein ID WP_066451866.1
Coordinates 3657202..3657483 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0X8HGZ7
Locus tag LOKO_RS16905 Protein ID WP_066451865.1
Coordinates 3656874..3657176 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOKO_RS16895 3654436..3655590 - 1155 WP_201025424.1 glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit -
LOKO_RS16900 3656018..3656821 - 804 WP_066451864.1 4-hydroxy-tetrahydrodipicolinate reductase -
LOKO_RS16905 3656874..3657176 - 303 WP_066451865.1 HigA family addiction module antidote protein Antitoxin
LOKO_RS16910 3657202..3657483 - 282 WP_066451866.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LOKO_RS16915 3657594..3658466 - 873 WP_066451867.1 DMT family transporter -
LOKO_RS16920 3658502..3658783 - 282 WP_066451868.1 DUF2218 domain-containing protein -
LOKO_RS19005 3658929..3659873 + 945 WP_158509958.1 HNH endonuclease -
LOKO_RS16930 3659943..3661097 - 1155 WP_066451870.1 molecular chaperone DnaJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3650624..3890825 240201


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-93)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-92)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10863.25 Da        Isoelectric Point: 8.7671

>T60418 WP_066451866.1 NZ_CP014226:c3657483-3657202 [Halomonas chromatireducens]
MIESFACKRTQRLFEGAGCSNSFRAFRRQAERKLQLLDSATTLDFLRSPPGNRLEALRGDRQGQYSIRINDQWRICFRFE
NGAAFDVEIVDYH

Download         Length: 282 bp

>T60418 NZ_CP014226:c3657483-3657202 [Halomonas chromatireducens]
ATGATCGAGTCCTTTGCCTGCAAGCGAACGCAGCGACTTTTCGAGGGGGCGGGGTGCTCCAACTCATTTCGTGCCTTCCG
GCGCCAGGCCGAGCGCAAGCTCCAATTGCTGGATTCGGCGACTACGCTGGATTTCTTGCGCTCGCCACCCGGTAACCGTC
TCGAGGCGCTACGCGGTGACAGGCAGGGGCAGTACAGCATTCGTATCAACGATCAGTGGAGAATCTGCTTTCGCTTCGAG
AACGGGGCCGCCTTCGATGTCGAAATCGTGGATTATCACTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11396.05 Da        Isoelectric Point: 6.7290

>AT60418 WP_066451865.1 NZ_CP014226:c3657176-3656874 [Halomonas chromatireducens]
MIKNGMRPIHPGEMLREDYLEPLGMSVNALARELRVPSTRLHEIVKERRAISADTALRLARYFGGDAQSWLNLQAAYELK
RVELDHGDEIERSVLPRDAA

Download         Length: 303 bp

>AT60418 NZ_CP014226:c3657176-3656874 [Halomonas chromatireducens]
ATGATCAAGAATGGCATGCGACCTATCCATCCAGGCGAGATGCTGCGGGAAGACTATCTGGAGCCGCTGGGGATGAGCGT
CAACGCCCTGGCACGTGAGCTGCGTGTGCCCTCGACCCGGTTGCACGAGATCGTCAAGGAGCGCCGTGCGATTTCGGCGG
ATACAGCCCTGCGTCTGGCGCGTTATTTCGGTGGTGATGCCCAGTCGTGGCTGAACCTTCAGGCAGCGTATGAGCTCAAG
AGAGTCGAGCTCGATCATGGCGACGAGATCGAGCGCAGCGTATTGCCACGAGATGCAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0X8HH20

Antitoxin

Source ID Structure
AlphaFold DB A0A0X8HGZ7

References