Detailed information of TA system TA060399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3090746..3091440
• Replicon: chromosome
• Accession: NZ_CP014225
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: AW869_RS15150
• Protein ID: WP_001263489.1
• Coordinates: 3091042..3091440 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AW869_RS15145
• Protein ID: WP_000554758.1
• Coordinates: 3090746..3091039 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AW869_RS15125	3086378..3086875	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
AW869_RS15130	3087099..3088811	-	1713	Protein_2860	flagellar biosynthesis protein FlhA	-
AW869_RS15135	3088783..3089568	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
AW869_RS15140	3089639..3090694	+	1056	WP_001226164.1	DNA polymerase IV	-
AW869_RS15145	3090746..3091039	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AW869_RS15150	3091042..3091440	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AW869_RS15155	3091450..3091902	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
AW869_RS15160	3092220..3092426	+	207	Protein_2866	RtcB family protein	-
AW869_RS15165	3092422..3092943	+	522	Protein_2867	peptide chain release factor H	-
AW869_RS15170	3093000..3094457	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
AW869_RS15175	3094718..3095176	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	3095772..3095852	+	81	NuclAT_12	-	-
-	3095772..3095852	+	81	NuclAT_12	-	-
-	3095772..3095852	+	81	NuclAT_12	-	-
-	3095772..3095852	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T60399 WP_001263489.1 NZ_CP014225:3091042-3091440 [Escherichia coli str. K-12 substr. MG1655]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T60399 NZ_CP014225:3091042-3091440 [Escherichia coli str. K-12 substr. MG1655]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT60399 WP_000554758.1 NZ_CP014225:3090746-3091039 [Escherichia coli str. K-12 substr. MG1655]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT60399 NZ_CP014225:3090746-3091039 [Escherichia coli str. K-12 substr. MG1655]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3