Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3084702..3085243 Replicon chromosome
Accession NZ_CP014225
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag AW869_RS15110 Protein ID WP_000615983.1
Coordinates 3084702..3084980 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag AW869_RS15115 Protein ID WP_000729703.1
Coordinates 3084983..3085243 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AW869_RS15095 3082284..3082862 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AW869_RS15100 3083068..3083835 + 768 WP_000333380.1 class II glutamine amidotransferase -
AW869_RS15105 3083806..3084546 - 741 WP_001225679.1 murein L,D-transpeptidase -
AW869_RS15110 3084702..3084980 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AW869_RS15115 3084983..3085243 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AW869_RS15120 3085453..3086202 + 750 WP_000056849.1 C40 family peptidase -
AW869_RS15125 3086378..3086875 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
AW869_RS15130 3087099..3088811 - 1713 Protein_2860 flagellar biosynthesis protein FlhA -
AW869_RS15135 3088783..3089568 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T60398 WP_000615983.1 NZ_CP014225:c3084980-3084702 [Escherichia coli str. K-12 substr. MG1655]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T60398 NZ_CP014225:c3084980-3084702 [Escherichia coli str. K-12 substr. MG1655]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT60398 WP_000729703.1 NZ_CP014225:c3085243-3084983 [Escherichia coli str. K-12 substr. MG1655]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT60398 NZ_CP014225:c3085243-3084983 [Escherichia coli str. K-12 substr. MG1655]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References