Detailed information of TA system TA060386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 1365051..1365744
• Replicon: chromosome
• Accession: NZ_CP014225
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: AW869_RS06705
• Protein ID: WP_000415584.1
• Coordinates: 1365448..1365744 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: AW869_RS06700
• Protein ID: WP_000650107.1
• Coordinates: 1365051..1365446 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AW869_RS06690	1360915..1363173	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
AW869_RS06695	1363311..1364918	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
AW869_RS06700	1365051..1365446	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
AW869_RS06705	1365448..1365744	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
AW869_RS06710	1365949..1366431	-	483	WP_000183505.1	transcriptional regulator	-
AW869_RS06715	1366484..1366876	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
AW869_RS06720	1367028..1367687	+	660	WP_001221495.1	two-component system response regulator QseB	-
AW869_RS06725	1367684..1369033	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
AW869_RS06730	1369079..1369411	-	333	WP_000917684.1	DUF2645 family protein	-
AW869_RS06735	1369730..1370311	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
AW869_RS06740	1370342..1370656	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T60386 WP_000415584.1 NZ_CP014225:c1365744-1365448 [Escherichia coli str. K-12 substr. MG1655]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T60386 NZ_CP014225:c1365744-1365448 [Escherichia coli str. K-12 substr. MG1655]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT60386 WP_000650107.1 NZ_CP014225:c1365446-1365051 [Escherichia coli str. K-12 substr. MG1655]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT60386 NZ_CP014225:c1365446-1365051 [Escherichia coli str. K-12 substr. MG1655]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65