Detailed information of TA system TA060368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1416728..1417331
• Replicon: chromosome
• Accession: NZ_CP014222
• Organism: Janthinobacterium sp. B9-8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0X8GN35
• Locus tag: VN23_RS06380
• Protein ID: WP_046352024.1
• Coordinates: 1417020..1417331 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: VN23_RS06375
• Protein ID: WP_046352025.1
• Coordinates: 1416728..1417018 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VN23_RS06340	1412156..1412407	-	252	WP_156455130.1	hypothetical protein	-
VN23_RS06345	1412412..1412639	-	228	WP_046352031.1	hypothetical protein	-
VN23_RS06350	1412632..1414530	-	1899	WP_046352030.1	replication endonuclease	-
VN23_RS06355	1414527..1414907	-	381	WP_046352029.1	hypothetical protein	-
VN23_RS06360	1415038..1415460	+	423	WP_156455131.1	helix-turn-helix domain-containing protein	-
VN23_RS06365	1415654..1416097	+	444	WP_046352027.1	hypothetical protein	-
VN23_RS06370	1416291..1416731	-	441	WP_046352026.1	hypothetical protein	-
VN23_RS06375	1416728..1417018	-	291	WP_046352025.1	helix-turn-helix domain-containing protein	Antitoxin
VN23_RS06380	1417020..1417331	-	312	WP_046352024.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VN23_RS06385	1417515..1418498	-	984	WP_052746593.1	hypothetical protein	-
VN23_RS06390	1418495..1418800	-	306	WP_046352023.1	DUF2523 domain-containing protein	-
VN23_RS06395	1418810..1420171	-	1362	WP_046352022.1	hypothetical protein	-
VN23_RS06400	1420288..1420497	-	210	WP_052746592.1	hypothetical protein	-
VN23_RS06405	1420490..1420711	-	222	WP_046352020.1	hypothetical protein	-
VN23_RS06410	1420716..1421921	-	1206	WP_046352019.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1383521..1435968	52447

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (40-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-80)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11741.53 Da
• Isoelectric Point: 8.3800

>T60368 WP_046352024.1 NZ_CP014222:c1417331-1417020 [Janthinobacterium sp. B9-8]
MEFIETPTFTRLLKALLTDDEYRGLQNELVENPARGDVIKGGGGIRKIRYAVTGRGKSGGVRAIYYWVREDHQIYMLLIY
PKSKKDTLTDAETAILHDLVKEL

Nucleotide

• Length: 312 bp

>T60368 NZ_CP014222:c1417331-1417020 [Janthinobacterium sp. B9-8]
ATGGAATTTATCGAAACCCCAACATTTACCCGCTTGCTGAAAGCACTTTTAACAGACGATGAATACAGAGGCTTACAAAA
TGAATTGGTAGAAAACCCCGCGCGTGGCGATGTTATTAAGGGCGGGGGAGGTATTCGTAAAATCCGTTATGCCGTCACAG
GCCGCGGTAAAAGTGGTGGAGTCAGGGCGATTTATTACTGGGTTAGGGAGGACCATCAGATTTACATGTTACTCATTTAC
CCCAAGTCTAAAAAAGACACGCTAACTGACGCTGAGACCGCGATTTTGCACGATTTAGTAAAGGAGCTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10565.03 Da
• Isoelectric Point: 8.8766

>AT60368 WP_046352025.1 NZ_CP014222:c1417018-1416728 [Janthinobacterium sp. B9-8]
MEPSLFEDLVQSLKEAKSIASGENQTARRFEVLPPDVRAIRAQTGLSQGEFAGLMRVSVKTLQNWEQHRRNPTGPAAALL
QIVSKSPSLALESLHQ

Nucleotide

• Length: 291 bp

>AT60368 NZ_CP014222:c1417018-1416728 [Janthinobacterium sp. B9-8]
ATGGAACCGTCACTTTTCGAGGACTTGGTGCAGAGTCTTAAAGAAGCAAAAAGCATTGCATCCGGGGAGAACCAAACAGC
ACGTCGTTTTGAGGTTTTGCCGCCCGATGTGCGAGCGATCAGAGCACAGACAGGATTGTCTCAGGGCGAGTTTGCGGGGC
TGATGCGTGTAAGCGTAAAGACGCTTCAAAATTGGGAACAGCACCGGCGTAACCCGACCGGCCCAGCAGCAGCATTGCTG
CAAATTGTTTCGAAGTCGCCAAGCTTGGCGCTTGAGTCCTTGCATCAATGA

Structures

Toxin

• AlphaFold DB: A0A0X8GN35