Detailed information of TA system TA060367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 8152..8810
• Replicon: plasmid unnamed1
• Accession: NZ_CP014216
• Organism: Acinetobacter baumannii strain YU-R612

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AXA63_RS19715
• Protein ID: WP_000312250.1
• Coordinates: 8451..8810 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AXA63_RS19710
• Protein ID: WP_001096429.1
• Coordinates: 8152..8451 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA63_RS19670	3319..3948	+	630	WP_002081923.1	hypothetical protein	-
AXA63_RS19675	3988..4497	+	510	WP_001043199.1	hypothetical protein	-
AXA63_RS19680	4579..5091	+	513	WP_000840065.1	hypothetical protein	-
AXA63_RS19685	5135..5476	+	342	WP_002001471.1	hypothetical protein	-
AXA63_RS19690	5513..6241	+	729	WP_001022819.1	hypothetical protein	-
AXA63_RS20990	6476..7029	+	554	Protein_10	hypothetical protein	-
AXA63_RS19705	7079..7615	+	537	WP_060853649.1	hypothetical protein	-
AXA63_RS19710	8152..8451	-	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
AXA63_RS19715	8451..8810	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXA63_RS19720	9011..9577	+	567	WP_031948745.1	hypothetical protein	-
AXA63_RS19725	9626..9808	+	183	WP_000373384.1	hypothetical protein	-
AXA63_RS19730	9875..10573	+	699	WP_000873188.1	hypothetical protein	-
AXA63_RS19735	10712..11095	+	384	WP_000654347.1	hypothetical protein	-
AXA63_RS19740	11162..11920	+	759	WP_060853650.1	hypothetical protein	-
AXA63_RS19745	11972..12307	+	336	WP_000654055.1	hypothetical protein	-
AXA63_RS19750	13172..13486	+	315	WP_000708713.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..74241	74241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T60367 WP_000312250.1 NZ_CP014216:c8810-8451 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T60367 NZ_CP014216:c8810-8451 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT60367 WP_001096429.1 NZ_CP014216:c8451-8152 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT60367 NZ_CP014216:c8451-8152 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA