Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) pmenTA/-
Location 615763..617495 Replicon chromosome
Accession NC_009439
Organism Pseudomonas mendocina ymp

Toxin (Protein)


Gene name pmenT Uniprot ID -
Locus tag PMEN_RS02800 Protein ID WP_011920781.1
Coordinates 615763..616416 (+) Length 218 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID -
Locus tag PMEN_RS02805 Protein ID WP_011920782.1
Coordinates 616413..617495 (+) Length 361 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PMEN_RS02790 (Pmen_0563) 611804..613933 + 2130 Protein_566 SAM-dependent DNA methyltransferase -
PMEN_RS02795 (Pmen_0564) 614523..615770 + 1248 WP_011920780.1 restriction endonuclease subunit S -
PMEN_RS02800 (Pmen_0565) 615763..616416 + 654 WP_011920781.1 DUF4433 domain-containing protein Toxin
PMEN_RS02805 (Pmen_0566) 616413..617495 + 1083 WP_011920782.1 macro domain-containing protein Antitoxin
PMEN_RS02810 (Pmen_0567) 617504..620767 + 3264 WP_011920783.1 type I restriction endonuclease subunit R -
PMEN_RS02815 (Pmen_0568) 620768..621475 + 708 WP_011920784.1 M48 family metallopeptidase -
PMEN_RS02820 (Pmen_0569) 621578..622426 + 849 WP_011920785.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(16-216)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 218 a.a.        Molecular weight: 25180.81 Da        Isoelectric Point: 7.1588

>T6034 WP_011920781.1 NC_009439:615763-616416 [Pseudomonas mendocina ymp]
MPNHYPNLNPEKALIWRIVHRDNLVWILDNGLHCASANVQAPNYVVIGNVDLISRRRERTVPVAPGGTLSDYVPFYFTPF
SPMMYNIHTGRGEVQQRHNEEICILVSSLRRVHELGLGFAFTDRHAYTPLARYFNELDLLEQLDWPLLQARNFSRNPDDP
EQIERYQAEALVHRYLPIEGLLGVVCFTQAVKDRLDAQAQAKGLKLDIRVMPQWYFR

Download         Length: 654 bp

>T6034 NC_009439:615763-616416 [Pseudomonas mendocina ymp]
ATGCCTAACCACTACCCCAACCTGAATCCGGAGAAGGCGCTGATCTGGCGTATTGTCCACCGCGATAATCTGGTGTGGAT
TCTGGATAACGGGCTGCACTGCGCCTCGGCGAATGTGCAGGCCCCGAATTATGTGGTGATTGGCAACGTCGATCTGATCA
GCCGGAGGCGTGAGCGCACGGTGCCGGTTGCGCCGGGCGGCACCTTGTCCGACTACGTGCCGTTCTATTTCACCCCGTTC
TCGCCCATGATGTACAACATCCATACTGGTCGCGGTGAGGTGCAGCAGCGGCACAACGAGGAAATCTGCATCCTGGTGTC
GAGCCTGCGTCGAGTGCATGAGCTAGGGTTGGGTTTTGCCTTTACGGATCGGCATGCCTACACCCCGCTGGCCAGGTACT
TCAATGAGCTGGATTTGCTTGAACAGTTGGATTGGCCGTTGCTGCAAGCGCGCAATTTTTCGCGCAACCCGGATGATCCG
GAGCAGATCGAGCGATACCAGGCCGAAGCCCTGGTACACCGCTATTTGCCCATAGAGGGCTTGCTCGGCGTGGTGTGTTT
TACCCAAGCCGTGAAAGATCGACTGGATGCACAGGCCCAGGCTAAAGGCTTGAAACTGGATATACGTGTTATGCCTCAGT
GGTACTTCCGATGA

Antitoxin


Download         Length: 361 a.a.        Molecular weight: 40518.53 Da        Isoelectric Point: 5.9520

>AT6034 WP_011920782.1 NC_009439:616413-617495 [Pseudomonas mendocina ymp]
MIRFTQGNLLEAKVDALVNTVNTVGVMGKGIALMFKERFAENYRLYAAACKAKQVETGKMFVTPVQELDGPRWIVNFPTK
RHWKAPSKMEWVVEGLTDLRTFILDNHIESIAVPPLGAGNGGLEWMAVREQIEAVLGDLHAEILVFEPTSQYQNVAKRSG
VEKLTPARALIAELVRRYWVLGMECSFLEVQKLAWFLERGIERFSPADNPLNLQFVAHKYGPYANRLEHLLNNLDGSYLH
AEKRISDSDPLDVIWFDDERKAYLQAYLNTESKPYLEALERTADLIDGFESPFGMELLATVDWLLAKEHVEATVPAVRQA
LRKWGGGPGAATRKDRLFDDRALAIALERLTQSGFSAVAA

Download         Length: 1083 bp

>AT6034 NC_009439:616413-617495 [Pseudomonas mendocina ymp]
ATGATTAGATTTACACAAGGCAATTTGCTGGAGGCCAAGGTCGATGCCTTGGTCAATACCGTCAACACTGTCGGCGTGAT
GGGCAAAGGTATTGCCCTGATGTTCAAGGAGCGCTTTGCTGAGAACTACCGGCTATACGCGGCAGCCTGCAAAGCCAAAC
AGGTTGAAACTGGCAAGATGTTTGTTACCCCGGTGCAGGAACTGGATGGTCCGCGCTGGATCGTCAACTTCCCCACCAAG
CGGCATTGGAAGGCTCCTTCCAAGATGGAATGGGTCGTTGAGGGGTTGACTGATCTGCGTACCTTTATCCTCGATAATCA
TATCGAGTCGATTGCCGTACCTCCGCTGGGGGCCGGTAATGGGGGGCTGGAATGGATGGCTGTACGCGAGCAGATCGAGG
CTGTTCTGGGCGACCTGCATGCTGAGATTTTGGTGTTTGAACCCACTTCGCAGTATCAGAACGTTGCCAAGCGCTCAGGG
GTAGAGAAGTTGACGCCGGCCCGTGCCTTGATCGCCGAGTTGGTAAGGCGCTACTGGGTATTGGGTATGGAATGCAGCTT
TCTGGAAGTGCAGAAGCTGGCCTGGTTTCTGGAGCGCGGTATCGAGCGATTTTCGCCTGCAGACAATCCACTGAACCTTC
AGTTTGTTGCCCATAAATACGGGCCCTATGCCAACCGTCTTGAGCATTTGCTGAACAATCTGGATGGCAGCTATCTGCAT
GCCGAAAAACGTATCAGCGATTCGGACCCGCTTGATGTCATCTGGTTTGATGACGAGCGTAAGGCCTATTTGCAGGCTTA
CTTAAACACTGAGTCAAAGCCCTATCTTGAGGCCTTGGAACGTACAGCAGACCTTATCGACGGCTTTGAGTCGCCCTTTG
GTATGGAGCTTTTGGCGACCGTAGATTGGCTGTTGGCCAAGGAGCATGTTGAGGCTACGGTGCCGGCTGTACGGCAAGCG
CTAAGGAAGTGGGGCGGTGGGCCAGGTGCTGCTACACGCAAGGATCGCTTGTTTGATGATCGTGCGTTGGCCATTGCCTT
GGAGCGCCTGACGCAGTCGGGTTTTAGTGCTGTAGCGGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10056 Thermus sp. 2.9

41.935

88.995

0.373

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT6240 Mycobacterium tuberculosis H37Rv

37.158

100

0.386

Multiple sequence alignment    




Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References

(1) Hila Sberro et al. (2013) Discovery of functional toxin/antitoxin systems in bacteria by shotgun cloning. Molecular Cell 50(1):136-48. [PubMed:23478446] experimental


experimental experimental literature