Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | pmenTA/- |
| Location | 615763..617495 | Replicon | chromosome |
| Accession | NC_009439 | ||
| Organism | Pseudomonas mendocina ymp | ||
Toxin (Protein)
| Gene name | pmenT | Uniprot ID | - |
| Locus tag | PMEN_RS02800 | Protein ID | WP_011920781.1 |
| Coordinates | 615763..616416 (+) | Length | 218 a.a. |
Antitoxin (Protein)
| Gene name | pmenA | Uniprot ID | - |
| Locus tag | PMEN_RS02805 | Protein ID | WP_011920782.1 |
| Coordinates | 616413..617495 (+) | Length | 361 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PMEN_RS02790 (Pmen_0563) | 611804..613933 | + | 2130 | Protein_566 | SAM-dependent DNA methyltransferase | - |
| PMEN_RS02795 (Pmen_0564) | 614523..615770 | + | 1248 | WP_011920780.1 | restriction endonuclease subunit S | - |
| PMEN_RS02800 (Pmen_0565) | 615763..616416 | + | 654 | WP_011920781.1 | DUF4433 domain-containing protein | Toxin |
| PMEN_RS02805 (Pmen_0566) | 616413..617495 | + | 1083 | WP_011920782.1 | macro domain-containing protein | Antitoxin |
| PMEN_RS02810 (Pmen_0567) | 617504..620767 | + | 3264 | WP_011920783.1 | type I restriction endonuclease subunit R | - |
| PMEN_RS02815 (Pmen_0568) | 620768..621475 | + | 708 | WP_011920784.1 | M48 family metallopeptidase | - |
| PMEN_RS02820 (Pmen_0569) | 621578..622426 | + | 849 | WP_011920785.1 | 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 218 a.a. Molecular weight: 25180.81 Da Isoelectric Point: 7.1588
>T6034 WP_011920781.1 NC_009439:615763-616416 [Pseudomonas mendocina ymp]
MPNHYPNLNPEKALIWRIVHRDNLVWILDNGLHCASANVQAPNYVVIGNVDLISRRRERTVPVAPGGTLSDYVPFYFTPF
SPMMYNIHTGRGEVQQRHNEEICILVSSLRRVHELGLGFAFTDRHAYTPLARYFNELDLLEQLDWPLLQARNFSRNPDDP
EQIERYQAEALVHRYLPIEGLLGVVCFTQAVKDRLDAQAQAKGLKLDIRVMPQWYFR
MPNHYPNLNPEKALIWRIVHRDNLVWILDNGLHCASANVQAPNYVVIGNVDLISRRRERTVPVAPGGTLSDYVPFYFTPF
SPMMYNIHTGRGEVQQRHNEEICILVSSLRRVHELGLGFAFTDRHAYTPLARYFNELDLLEQLDWPLLQARNFSRNPDDP
EQIERYQAEALVHRYLPIEGLLGVVCFTQAVKDRLDAQAQAKGLKLDIRVMPQWYFR
Download Length: 654 bp
>T6034 NC_009439:615763-616416 [Pseudomonas mendocina ymp]
ATGCCTAACCACTACCCCAACCTGAATCCGGAGAAGGCGCTGATCTGGCGTATTGTCCACCGCGATAATCTGGTGTGGAT
TCTGGATAACGGGCTGCACTGCGCCTCGGCGAATGTGCAGGCCCCGAATTATGTGGTGATTGGCAACGTCGATCTGATCA
GCCGGAGGCGTGAGCGCACGGTGCCGGTTGCGCCGGGCGGCACCTTGTCCGACTACGTGCCGTTCTATTTCACCCCGTTC
TCGCCCATGATGTACAACATCCATACTGGTCGCGGTGAGGTGCAGCAGCGGCACAACGAGGAAATCTGCATCCTGGTGTC
GAGCCTGCGTCGAGTGCATGAGCTAGGGTTGGGTTTTGCCTTTACGGATCGGCATGCCTACACCCCGCTGGCCAGGTACT
TCAATGAGCTGGATTTGCTTGAACAGTTGGATTGGCCGTTGCTGCAAGCGCGCAATTTTTCGCGCAACCCGGATGATCCG
GAGCAGATCGAGCGATACCAGGCCGAAGCCCTGGTACACCGCTATTTGCCCATAGAGGGCTTGCTCGGCGTGGTGTGTTT
TACCCAAGCCGTGAAAGATCGACTGGATGCACAGGCCCAGGCTAAAGGCTTGAAACTGGATATACGTGTTATGCCTCAGT
GGTACTTCCGATGA
ATGCCTAACCACTACCCCAACCTGAATCCGGAGAAGGCGCTGATCTGGCGTATTGTCCACCGCGATAATCTGGTGTGGAT
TCTGGATAACGGGCTGCACTGCGCCTCGGCGAATGTGCAGGCCCCGAATTATGTGGTGATTGGCAACGTCGATCTGATCA
GCCGGAGGCGTGAGCGCACGGTGCCGGTTGCGCCGGGCGGCACCTTGTCCGACTACGTGCCGTTCTATTTCACCCCGTTC
TCGCCCATGATGTACAACATCCATACTGGTCGCGGTGAGGTGCAGCAGCGGCACAACGAGGAAATCTGCATCCTGGTGTC
GAGCCTGCGTCGAGTGCATGAGCTAGGGTTGGGTTTTGCCTTTACGGATCGGCATGCCTACACCCCGCTGGCCAGGTACT
TCAATGAGCTGGATTTGCTTGAACAGTTGGATTGGCCGTTGCTGCAAGCGCGCAATTTTTCGCGCAACCCGGATGATCCG
GAGCAGATCGAGCGATACCAGGCCGAAGCCCTGGTACACCGCTATTTGCCCATAGAGGGCTTGCTCGGCGTGGTGTGTTT
TACCCAAGCCGTGAAAGATCGACTGGATGCACAGGCCCAGGCTAAAGGCTTGAAACTGGATATACGTGTTATGCCTCAGT
GGTACTTCCGATGA
Antitoxin
Download Length: 361 a.a. Molecular weight: 40518.53 Da Isoelectric Point: 5.9520
>AT6034 WP_011920782.1 NC_009439:616413-617495 [Pseudomonas mendocina ymp]
MIRFTQGNLLEAKVDALVNTVNTVGVMGKGIALMFKERFAENYRLYAAACKAKQVETGKMFVTPVQELDGPRWIVNFPTK
RHWKAPSKMEWVVEGLTDLRTFILDNHIESIAVPPLGAGNGGLEWMAVREQIEAVLGDLHAEILVFEPTSQYQNVAKRSG
VEKLTPARALIAELVRRYWVLGMECSFLEVQKLAWFLERGIERFSPADNPLNLQFVAHKYGPYANRLEHLLNNLDGSYLH
AEKRISDSDPLDVIWFDDERKAYLQAYLNTESKPYLEALERTADLIDGFESPFGMELLATVDWLLAKEHVEATVPAVRQA
LRKWGGGPGAATRKDRLFDDRALAIALERLTQSGFSAVAA
MIRFTQGNLLEAKVDALVNTVNTVGVMGKGIALMFKERFAENYRLYAAACKAKQVETGKMFVTPVQELDGPRWIVNFPTK
RHWKAPSKMEWVVEGLTDLRTFILDNHIESIAVPPLGAGNGGLEWMAVREQIEAVLGDLHAEILVFEPTSQYQNVAKRSG
VEKLTPARALIAELVRRYWVLGMECSFLEVQKLAWFLERGIERFSPADNPLNLQFVAHKYGPYANRLEHLLNNLDGSYLH
AEKRISDSDPLDVIWFDDERKAYLQAYLNTESKPYLEALERTADLIDGFESPFGMELLATVDWLLAKEHVEATVPAVRQA
LRKWGGGPGAATRKDRLFDDRALAIALERLTQSGFSAVAA
Download Length: 1083 bp
>AT6034 NC_009439:616413-617495 [Pseudomonas mendocina ymp]
ATGATTAGATTTACACAAGGCAATTTGCTGGAGGCCAAGGTCGATGCCTTGGTCAATACCGTCAACACTGTCGGCGTGAT
GGGCAAAGGTATTGCCCTGATGTTCAAGGAGCGCTTTGCTGAGAACTACCGGCTATACGCGGCAGCCTGCAAAGCCAAAC
AGGTTGAAACTGGCAAGATGTTTGTTACCCCGGTGCAGGAACTGGATGGTCCGCGCTGGATCGTCAACTTCCCCACCAAG
CGGCATTGGAAGGCTCCTTCCAAGATGGAATGGGTCGTTGAGGGGTTGACTGATCTGCGTACCTTTATCCTCGATAATCA
TATCGAGTCGATTGCCGTACCTCCGCTGGGGGCCGGTAATGGGGGGCTGGAATGGATGGCTGTACGCGAGCAGATCGAGG
CTGTTCTGGGCGACCTGCATGCTGAGATTTTGGTGTTTGAACCCACTTCGCAGTATCAGAACGTTGCCAAGCGCTCAGGG
GTAGAGAAGTTGACGCCGGCCCGTGCCTTGATCGCCGAGTTGGTAAGGCGCTACTGGGTATTGGGTATGGAATGCAGCTT
TCTGGAAGTGCAGAAGCTGGCCTGGTTTCTGGAGCGCGGTATCGAGCGATTTTCGCCTGCAGACAATCCACTGAACCTTC
AGTTTGTTGCCCATAAATACGGGCCCTATGCCAACCGTCTTGAGCATTTGCTGAACAATCTGGATGGCAGCTATCTGCAT
GCCGAAAAACGTATCAGCGATTCGGACCCGCTTGATGTCATCTGGTTTGATGACGAGCGTAAGGCCTATTTGCAGGCTTA
CTTAAACACTGAGTCAAAGCCCTATCTTGAGGCCTTGGAACGTACAGCAGACCTTATCGACGGCTTTGAGTCGCCCTTTG
GTATGGAGCTTTTGGCGACCGTAGATTGGCTGTTGGCCAAGGAGCATGTTGAGGCTACGGTGCCGGCTGTACGGCAAGCG
CTAAGGAAGTGGGGCGGTGGGCCAGGTGCTGCTACACGCAAGGATCGCTTGTTTGATGATCGTGCGTTGGCCATTGCCTT
GGAGCGCCTGACGCAGTCGGGTTTTAGTGCTGTAGCGGCTTGA
ATGATTAGATTTACACAAGGCAATTTGCTGGAGGCCAAGGTCGATGCCTTGGTCAATACCGTCAACACTGTCGGCGTGAT
GGGCAAAGGTATTGCCCTGATGTTCAAGGAGCGCTTTGCTGAGAACTACCGGCTATACGCGGCAGCCTGCAAAGCCAAAC
AGGTTGAAACTGGCAAGATGTTTGTTACCCCGGTGCAGGAACTGGATGGTCCGCGCTGGATCGTCAACTTCCCCACCAAG
CGGCATTGGAAGGCTCCTTCCAAGATGGAATGGGTCGTTGAGGGGTTGACTGATCTGCGTACCTTTATCCTCGATAATCA
TATCGAGTCGATTGCCGTACCTCCGCTGGGGGCCGGTAATGGGGGGCTGGAATGGATGGCTGTACGCGAGCAGATCGAGG
CTGTTCTGGGCGACCTGCATGCTGAGATTTTGGTGTTTGAACCCACTTCGCAGTATCAGAACGTTGCCAAGCGCTCAGGG
GTAGAGAAGTTGACGCCGGCCCGTGCCTTGATCGCCGAGTTGGTAAGGCGCTACTGGGTATTGGGTATGGAATGCAGCTT
TCTGGAAGTGCAGAAGCTGGCCTGGTTTCTGGAGCGCGGTATCGAGCGATTTTCGCCTGCAGACAATCCACTGAACCTTC
AGTTTGTTGCCCATAAATACGGGCCCTATGCCAACCGTCTTGAGCATTTGCTGAACAATCTGGATGGCAGCTATCTGCAT
GCCGAAAAACGTATCAGCGATTCGGACCCGCTTGATGTCATCTGGTTTGATGACGAGCGTAAGGCCTATTTGCAGGCTTA
CTTAAACACTGAGTCAAAGCCCTATCTTGAGGCCTTGGAACGTACAGCAGACCTTATCGACGGCTTTGAGTCGCCCTTTG
GTATGGAGCTTTTGGCGACCGTAGATTGGCTGTTGGCCAAGGAGCATGTTGAGGCTACGGTGCCGGCTGTACGGCAAGCG
CTAAGGAAGTGGGGCGGTGGGCCAGGTGCTGCTACACGCAAGGATCGCTTGTTTGATGATCGTGCGTTGGCCATTGCCTT
GGAGCGCCTGACGCAGTCGGGTTTTAGTGCTGTAGCGGCTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10056 | Thermus sp. 2.9 |
41.935 |
88.995 |
0.373 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6240 | Mycobacterium tuberculosis H37Rv |
37.158 |
100 |
0.386 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Hila Sberro et al. (2013) Discovery of functional toxin/antitoxin systems in bacteria by shotgun cloning. Molecular Cell 50(1):136-48. [PubMed:23478446]