10056

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) darTG/-
Location 71743..73734 Replicon chromosome
Accession NZ_JTJB01000003
Organism Thermus sp. 2.9

Toxin (Protein)


Gene name darT Uniprot ID A0A0B0SG80
Locus tag QT17_RS01925 Protein ID WP_039455677.1
Coordinates 72828..73457 (-) Length 210 a.a.

Antitoxin (Protein)


Gene name darG Uniprot ID A0A0B0SDG1
Locus tag QT17_RS01920 Protein ID WP_039455675.1
Coordinates 71743..72819 (-) Length 359 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QT17_RS14800 (QT17_01915) 69303..70763 + 1461 WP_039455673.1 hypothetical protein -
QT17_RS13005 70772..71746 + 975 WP_052155138.1 phage baseplate assembly protein V -
QT17_RS01920 (QT17_01925) 71743..72819 - 1077 WP_039455675.1 macro domain-containing protein Antitoxin
QT17_RS01925 (QT17_01930) 72828..73457 - 630 WP_039455677.1 DUF4433 domain-containing protein Toxin
QT17_RS01935 (QT17_01940) 73816..74076 + 261 WP_039455681.1 PAAR domain-containing protein -
QT17_RS14805 (QT17_01945) 74103..75380 + 1278 WP_039455683.1 hypothetical protein -
QT17_RS01945 (QT17_01950) 75377..76000 + 624 WP_039455685.1 hypothetical protein -
QT17_RS01950 (QT17_01955) 75997..77049 + 1053 WP_039455688.1 baseplate J/gp47 family protein -
QT17_RS01955 (QT17_01960) 77050..77832 + 783 WP_039455690.1 hypothetical protein -
QT17_RS13795 (QT17_01965) 77859..78242 + 384 WP_039455692.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(12-209)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 210 a.a.        Molecular weight: 24371.11 Da        Isoelectric Point: 9.6404

>T10056 WP_039455677.1 NZ_JTJB01000003:c73457-72828 [Thermus sp. 2.9]
MKRTYPEPTPIYHITHIDNLKGILRMGKLLAHNQSPPKQRSIAYAHIQERRNRAKVPQPPGGVLHDYVPFYFCPRSPMLY
AIYSGATEYQGGQEPILHLVSSAQAVHKAGLPFVFTDRHGVLSHARFFRQLEELAQLDWEAIQASYWADPPELREKKQAE
FLVYKAFPWALIEEIAVYSQRVGEEVLKILKQFPEARRPRVCIRKDWYY

Download         Length: 630 bp

>T10056 NZ_JTJB01000003:c73457-72828 [Thermus sp. 2.9]
ATGAAACGAACCTACCCTGAACCAACACCCATTTACCACATCACCCACATAGACAACTTGAAGGGTATTCTCCGTATGGG
CAAGCTTCTTGCCCACAACCAAAGCCCCCCTAAGCAACGAAGCATCGCCTACGCGCACATTCAGGAGCGCCGTAACCGAG
CCAAAGTGCCCCAACCTCCCGGGGGCGTTCTTCACGACTACGTTCCCTTCTACTTCTGCCCCCGCTCCCCCATGCTTTAC
GCCATTTACTCTGGCGCCACGGAATACCAGGGAGGGCAAGAACCCATCCTGCACTTGGTTTCCTCGGCTCAAGCGGTGCA
CAAGGCAGGGCTACCTTTTGTGTTCACTGACCGACATGGGGTCTTGAGCCACGCCAGGTTCTTTCGTCAGCTAGAAGAGC
TGGCTCAGCTGGACTGGGAAGCCATCCAAGCCTCCTACTGGGCCGATCCTCCGGAGTTGCGGGAAAAGAAGCAGGCGGAG
TTTCTGGTCTACAAGGCTTTCCCGTGGGCCCTAATAGAAGAAATAGCGGTTTACAGCCAAAGGGTTGGGGAAGAGGTTCT
TAAAATCCTCAAGCAGTTTCCAGAGGCACGCCGTCCCCGGGTGTGCATCCGGAAGGACTGGTATTATTAG

Antitoxin


Download         Length: 359 a.a.        Molecular weight: 7921.18 Da        Isoelectric Point: 10.0406

>AT10056 WP_039455675.1 NZ_JTJB01000003:c72819-71743 [Thermus sp. 2.9]
MIRFVQGDLLQAPAEALVNTVNTVGVMGKGVALQFKRAFPDNYEAYVKACERGEVQIGRIFVYDRGLLARPRYIFNFPTK
KHWRHPSRMAYVEEGLKDLVRVIQELGVRSVALPPLGAGNGGLPWPEVRRRIQEALEPLEDVEILVYEPQATEVPSITPL
SNKPRLTPPRAALLKLFGLYKTLGETLGRLEAQKLAYFLQEAGLNLRLDFARNQYGPYAEPLNHVLARLEGHYIQGYGDR
SSPSRIRLRANAFQEAVAYLADHPEADEAASRAAAWVEGFESPYGLELLATVHWAVRREGARTWPTLQHILAEWGPRKAN
IPQRDLQVALYYLLQRGALQPEEWSDTVELPKDAPQPA

Download         Length: 1077 bp

>AT10056 NZ_JTJB01000003:c72819-71743 [Thermus sp. 2.9]
ATGATCCGCTTTGTGCAAGGCGACCTCCTCCAAGCCCCGGCAGAGGCCTTGGTCAACACGGTGAACACCGTGGGGGTCAT
GGGAAAGGGGGTGGCTTTGCAGTTCAAGCGCGCCTTCCCTGACAACTACGAAGCTTATGTAAAAGCTTGTGAGCGAGGCG
AAGTGCAAATCGGTCGCATCTTTGTTTATGACCGCGGCCTTCTAGCCCGCCCACGCTACATTTTTAACTTCCCCACCAAA
AAACACTGGCGCCACCCCTCACGGATGGCGTACGTGGAGGAGGGCCTAAAAGATTTGGTTCGGGTAATCCAAGAGCTCGG
GGTACGCTCTGTGGCCCTACCCCCTCTGGGCGCAGGCAACGGGGGTCTCCCCTGGCCCGAGGTGCGCCGGCGCATTCAGG
AGGCTTTGGAACCTCTAGAGGACGTAGAAATCCTGGTCTACGAGCCCCAGGCCACTGAGGTCCCTTCCATCACCCCCCTC
TCGAACAAACCACGCCTCACCCCACCCCGGGCAGCCCTGCTCAAGCTCTTCGGCCTCTACAAAACCTTGGGGGAAACCTT
GGGGCGCCTCGAGGCGCAAAAACTGGCCTACTTCCTACAGGAGGCAGGCCTAAATCTGCGGCTGGATTTTGCCCGCAATC
AGTACGGCCCTTACGCCGAACCCCTTAACCACGTATTGGCCCGTCTCGAGGGGCACTACATCCAAGGCTACGGCGACCGG
AGCAGTCCCTCCCGCATCCGCCTCCGCGCAAACGCCTTCCAGGAAGCGGTGGCCTACTTGGCCGACCACCCCGAGGCGGA
CGAAGCCGCAAGCCGGGCTGCGGCCTGGGTAGAAGGCTTTGAAAGCCCGTACGGCTTGGAGCTTCTAGCCACAGTACACT
GGGCGGTACGCCGGGAAGGGGCTAGAACATGGCCAACCCTTCAACATATCCTGGCGGAATGGGGCCCCCGCAAAGCCAAC
ATCCCCCAACGGGATTTGCAGGTGGCCCTGTATTACCTGCTACAGCGGGGGGCCCTCCAGCCTGAGGAATGGTCCGATAC
GGTTGAGCTTCCCAAAGACGCTCCCCAGCCGGCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6034 Pseudomonas mendocina ymp

41.935

88.995

0.373
T6240 Mycobacterium tuberculosis H37Rv

30.882

97.608

0.301

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7OMX
PDB 7OMW
PDB 7OMZ
PDB 7ON0
PDB 7OMY
PDB 7OMV

Antitoxin

Source ID Structure

References

(1) Marion Schuller et al. (2021) Molecular basis for DarT ADP-ribosylation of a DNA base. Nature 596(7873):597-602. [PubMed:34408320] 3D_structure


3D_structure protein structure literature