Detailed information of TA system TA060325

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): bsrE-as-bsrE/-
• Location: 2044981..2045227
• Replicon: chromosome
• Accession: NZ_CP014166
• Organism: Bacillus subtilis subsp. subtilis strain CU1050

Toxin (Protein)

• Gene name: bsrE
• Uniprot ID: -
• Locus tag: AWM80_RS21905
• Protein ID: WP_109789044.1
• Coordinates: 2044981..2045073 (+)
• Length: 31 a.a.

Antitoxin (RNA)

Gene name	as-bsrE
Locus tag	-
Coordinates	2045054..2045227 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWM80_RS10285	2040140..2040475	+	336	WP_009967409.1	hypothetical protein	-
AWM80_RS10290	2040522..2044127	-	3606	WP_004399367.1	membrane protein	-
AWM80_RS10295	2044360..2044692	-	333	WP_026009814.1	hypothetical protein	-
AWM80_RS21905	2044981..2045073	+	93	WP_109789044.1	type I toxin-antitoxin system toxin BsrE	Toxin
-	2045054..2045227	-	174	-	-	Antitoxin
AWM80_RS10300	2045342..2046184	-	843	WP_003231327.1	hypothetical protein	-
AWM80_RS10305	2046384..2046842	-	459	WP_003231326.1	type II toxin-antitoxin system antitoxin YobK	-
AWM80_RS10310	2046852..2048654	-	1803	WP_004399481.1	type II toxin-antitoxin system toxin ribonuclease YobL	-
AWM80_RS10315	2048756..2049313	-	558	WP_004399321.1	SMI1/KNR4 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2031376..2054644	23268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (1-24)

Sequences

Toxin

Protein

• Length: 31 a.a.
• Molecular weight: 3358.16 Da
• Isoelectric Point: 4.1840

>T60325 WP_109789044.1 NZ_CP014166:2044981-2045073 [Bacillus subtilis subsp. subtilis]
MSTFQALMLMLAIGSFIIALLTYIEKIDLP

Nucleotide

• Length: 93 bp

>T60325 NZ_CP014166:2044981-2045073 [Bacillus subtilis subsp. subtilis]
ATGTCAACATTCCAGGCATTAATGCTAATGCTTGCAATCGGGTCGTTTATAATCGCCCTATTGACGTATATAGAAAAAAT
AGACCTCCCTTGA

Antitoxin

Nucleotide

• Length: 174 bp

>AT60325 NZ_CP014166:c2045227-2045054 [Bacillus subtilis subsp. subtilis]
TTACAATACATTACAGTGCATTTCTTTAATGAGAAATGCATAAAATAAAAAAGACCGAGGTGCTACCAACACCCCGGCTC
TGTACAAAAAGTTGCCCATCAAAGGGCTTGCTCGATGTGGTTCTAGGTAGGCCTATCCCTTCAGGTTCTGAGGCTCAAGG
GAGGTCTATTTTTT

Structures

Antitoxin