Detailed information of TA system TA060308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 4969524..4970079
• Replicon: chromosome
• Accession: NZ_CP014158
• Organism: Pseudomonas citronellolis strain P3B5

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: PcP3B5_RS21575
• Protein ID: WP_043270910.1
• Coordinates: 4969825..4970079 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A127MXF0
• Locus tag: PcP3B5_RS21570
• Protein ID: WP_061562366.1
• Coordinates: 4969524..4969796 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PcP3B5_RS21555	4965638..4966123	+	486	WP_009623540.1	GFA family protein	-
PcP3B5_RS21560	4966120..4968237	-	2118	WP_061562364.1	ergothioneine biosynthesis protein EgtB	-
PcP3B5_RS21565	4968505..4969380	+	876	WP_061562365.1	class A beta-lactamase	-
PcP3B5_RS21570	4969524..4969796	+	273	WP_061562366.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
PcP3B5_RS21575	4969825..4970079	+	255	WP_043270910.1	Txe/YoeB family addiction module toxin	Toxin
PcP3B5_RS21580	4970098..4970565	-	468	WP_061562367.1	RES family NAD+ phosphorylase	-
PcP3B5_RS21585	4970565..4971023	-	459	WP_061562368.1	MbcA/ParS/Xre antitoxin family protein	-
PcP3B5_RS21590	4971494..4971853	+	360	WP_009623553.1	multidrug/spermidine efflux SMR transporter subunit MdtJ	-
PcP3B5_RS21595	4971847..4972176	+	330	WP_061562369.1	multidrug/spermidine efflux SMR transporter subunit MdtI	-
PcP3B5_RS21600	4972547..4973947	-	1401	WP_043312797.1	GABA permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-82)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9794.02 Da
• Isoelectric Point: 7.2407

>T60308 WP_043270910.1 NZ_CP014158:4969825-4970079 [Pseudomonas citronellolis]
VAFTDHGWDDYQYWKGQDPKISATLDRLIGECLRTPFSGTGKPEALKGNLSGLWSRRITREHRLVYLFEDGQLFIVQCRY
HYDG

Nucleotide

• Length: 255 bp

>T60308 NZ_CP014158:4969825-4970079 [Pseudomonas citronellolis]
GTGGCTTTCACCGATCACGGCTGGGATGACTACCAATACTGGAAGGGCCAGGATCCGAAGATATCCGCAACCCTGGACCG
ACTCATCGGCGAATGCCTGCGTACCCCCTTCAGCGGCACCGGCAAGCCCGAAGCGCTGAAGGGTAATCTCAGCGGGCTCT
GGTCCCGCCGCATAACCCGCGAACACCGGCTGGTCTACCTGTTCGAAGACGGCCAACTCTTCATCGTGCAATGCCGCTAC
CACTACGACGGCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10234.51 Da
• Isoelectric Point: 4.6387

>AT60308 WP_061562366.1 NZ_CP014158:4969524-4969796 [Pseudomonas citronellolis]
MQILTFSQARASLKQAMDDVCRDHEPTVITRQRGEPVVLLSLEDYNGMRETMYLLQSSANAQRLHESIEQLRVGKSVPRE
LSISDDAEET

Nucleotide

• Length: 273 bp

>AT60308 NZ_CP014158:4969524-4969796 [Pseudomonas citronellolis]
ATGCAAATTCTGACATTCAGCCAAGCGCGAGCCAGCCTGAAACAGGCCATGGATGACGTATGCCGCGACCATGAGCCGAC
TGTCATCACCCGGCAAAGAGGCGAACCTGTGGTTCTGCTTTCGCTGGAGGACTACAACGGCATGCGCGAGACCATGTACC
TGCTGCAATCCTCGGCCAATGCACAACGGCTGCATGAATCCATCGAGCAGTTACGAGTCGGCAAGAGCGTGCCAAGGGAA
CTGAGCATCAGTGACGACGCAGAAGAAACGTAA

Structures

Antitoxin

• AlphaFold DB: A0A127MXF0