Detailed information of TA system TA060307

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3084352..3084965
• Replicon: chromosome
• Accession: NZ_CP014158
• Organism: Pseudomonas citronellolis strain P3B5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1S1GEA1
• Locus tag: PcP3B5_RS13645
• Protein ID: WP_043273738.1
• Coordinates: 3084352..3084648 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PcP3B5_RS13650
• Protein ID: WP_024128404.1
• Coordinates: 3084657..3084965 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PcP3B5_RS13635	3081563..3082729	-	1167	WP_043273736.1	efflux RND transporter periplasmic adaptor subunit	-
PcP3B5_RS13640	3082726..3084129	-	1404	WP_061561562.1	efflux transporter outer membrane subunit	-
PcP3B5_RS13645	3084352..3084648	+	297	WP_043273738.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PcP3B5_RS13650	3084657..3084965	+	309	WP_024128404.1	HigA family addiction module antidote protein	Antitoxin
PcP3B5_RS13655	3085173..3085880	-	708	WP_061561563.1	response regulator transcription factor	-
PcP3B5_RS13660	3085985..3086674	+	690	WP_058489774.1	response regulator transcription factor	-
PcP3B5_RS13665	3086652..3088031	+	1380	WP_061561564.1	HAMP domain-containing protein	-
PcP3B5_RS13670	3088218..3088784	-	567	WP_061561565.1	flavin reductase family protein	-
PcP3B5_RS13675	3088801..3089721	-	921	WP_061561566.1	bestrophin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11820.29 Da
• Isoelectric Point: 8.5982

>T60307 WP_043273738.1 NZ_CP014158:3084352-3084648 [Pseudomonas citronellolis]
MIGSFRDAWLEAFFVRDRMPRQIPADIQERLFRKLQMLDDATTDADLRSPPSNHFERLQGRLRAYHSIRVNRQWRLVFHW
DGEKGEASDVYLDNHSYR

Nucleotide

• Length: 297 bp

>T60307 NZ_CP014158:3084352-3084648 [Pseudomonas citronellolis]
ATGATCGGGAGTTTCAGGGATGCGTGGCTGGAAGCGTTCTTCGTGCGGGACAGGATGCCGCGGCAGATTCCCGCGGATAT
CCAGGAGCGCCTGTTCCGCAAGCTGCAGATGCTCGACGACGCCACCACGGACGCGGACTTGCGTAGCCCACCCAGCAACC
ATTTCGAGCGCTTGCAGGGGCGCCTGCGGGCATACCACTCGATCCGGGTGAATCGCCAATGGCGCCTGGTCTTTCATTGG
GATGGCGAAAAAGGCGAAGCCTCGGACGTCTACCTCGACAACCATTCGTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11471.20 Da
• Isoelectric Point: 11.1591

>AT60307 WP_024128404.1 NZ_CP014158:3084657-3084965 [Pseudomonas citronellolis]
VIITERKPVSVGEVLSSEFLDPLGLTQGRLAEAMGVQRKLVNELCNDRRAVTADTALMLARVFGNSAEFWLNLQRRNDLW
AALNSPERRARIERARPLNRAA

Nucleotide

• Length: 309 bp

>AT60307 NZ_CP014158:3084657-3084965 [Pseudomonas citronellolis]
GTGATCATCACCGAACGCAAGCCGGTCAGCGTCGGCGAGGTGCTCAGCAGCGAGTTCCTCGATCCGCTAGGCCTGACCCA
AGGCCGCCTCGCCGAAGCGATGGGGGTGCAGCGCAAGCTGGTCAACGAACTGTGCAACGACCGGAGGGCAGTGACGGCCG
ATACCGCCCTGATGCTGGCCCGGGTATTCGGCAACTCTGCGGAGTTCTGGCTCAACCTGCAGCGGCGCAACGACCTGTGG
GCGGCGTTGAATTCGCCAGAGCGACGTGCCCGCATAGAGCGGGCTCGGCCATTGAATCGGGCGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A1S1GEA1