Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 3055496..3056118 Replicon chromosome
Accession NZ_CP014158
Organism Pseudomonas citronellolis strain P3B5

Toxin (Protein)


Gene name higB Uniprot ID A0A127MSF6
Locus tag PcP3B5_RS13505 Protein ID WP_043317089.1
Coordinates 3055804..3056118 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W5IVT4
Locus tag PcP3B5_RS13500 Protein ID WP_009619925.1
Coordinates 3055496..3055801 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PcP3B5_RS13470 3050656..3050865 - 210 WP_009619863.1 DUF2945 domain-containing protein -
PcP3B5_RS13475 3051062..3052447 + 1386 WP_043317102.1 oxygen-independent coproporphyrinogen III oxidase -
PcP3B5_RS13480 3052542..3053390 - 849 WP_043317100.1 amidohydrolase family protein -
PcP3B5_RS13490 3054137..3054513 - 377 Protein_2703 integrase -
PcP3B5_RS31995 3054820..3055104 - 285 WP_203228552.1 hypothetical protein -
PcP3B5_RS13500 3055496..3055801 - 306 WP_009619925.1 helix-turn-helix domain-containing protein Antitoxin
PcP3B5_RS13505 3055804..3056118 - 315 WP_043317089.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PcP3B5_RS13510 3056198..3056380 - 183 WP_061561551.1 hypothetical protein -
PcP3B5_RS13520 3058674..3059024 - 351 WP_009619928.1 hypothetical protein -
PcP3B5_RS13525 3059456..3059881 + 426 WP_058489395.1 hypothetical protein -
PcP3B5_RS13530 3060187..3060606 - 420 WP_052267801.1 LytTR family transcriptional regulator DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-101)

Antitoxin

Cro/C1-type helix-turn-helix domain

(36-72)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11744.48 Da        Isoelectric Point: 9.4385

>T60306 WP_043317089.1 NZ_CP014158:c3056118-3055804 [Pseudomonas citronellolis]
MIFIETPVFTKRILALVDDETYRKLQEDLTLHPDAGAVIEGTGGVRKIRIAANGHGKRGGARVIYYHFTSASQIAFLLVY
DKASQEDLTADQKKVLRQIVENWR

Download         Length: 315 bp

>T60306 NZ_CP014158:c3056118-3055804 [Pseudomonas citronellolis]
ATGATCTTCATCGAAACGCCAGTCTTCACGAAGCGGATTCTCGCGCTAGTAGACGATGAGACCTATCGCAAGCTTCAGGA
AGACCTAACGCTCCACCCGGACGCCGGAGCAGTCATTGAAGGCACGGGTGGCGTGCGCAAGATTCGCATTGCTGCCAATG
GACACGGGAAAAGAGGCGGCGCCCGGGTCATCTACTACCACTTCACATCCGCCTCTCAAATTGCATTCCTGCTGGTCTAT
GACAAGGCATCGCAGGAGGACCTGACTGCCGATCAGAAAAAGGTACTTCGGCAGATCGTTGAGAACTGGAGATAG

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11385.90 Da        Isoelectric Point: 7.2080

>AT60306 WP_009619925.1 NZ_CP014158:c3055801-3055496 [Pseudomonas citronellolis]
MDKELFAELVESVTQMDEIVRGERQPSREFHIDAVKVREIRRATGLSQAKFARKINVAVGTLRNWEQGRRDPEGPARALL
RAIDNDPVHVLAALNGGPRED

Download         Length: 306 bp

>AT60306 NZ_CP014158:c3055801-3055496 [Pseudomonas citronellolis]
ATGGACAAAGAACTCTTCGCTGAACTGGTCGAAAGCGTGACCCAGATGGACGAGATCGTGCGCGGCGAACGTCAACCTTC
CCGCGAATTCCACATCGACGCCGTGAAGGTTCGCGAAATCCGCCGCGCTACTGGCCTGTCCCAGGCCAAATTCGCCCGGA
AAATTAATGTTGCTGTCGGCACGCTGCGCAATTGGGAGCAAGGGCGCCGCGATCCCGAGGGGCCTGCTCGCGCGCTGCTC
CGAGCCATCGATAATGATCCGGTCCATGTTCTAGCTGCCCTGAACGGCGGCCCGCGAGAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A127MSF6

Antitoxin

Source ID Structure
AlphaFold DB A0A127MSV9

References