Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-couple_hipB
Location 718912..719569 Replicon chromosome
Accession NZ_CP014144
Organism Streptococcus salivarius strain JF

Toxin (Protein)


Gene name higB Uniprot ID A0A3S4MPX5
Locus tag AWB63_RS03440 Protein ID WP_004182758.1
Coordinates 719216..719569 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A448A9W2
Locus tag AWB63_RS03435 Protein ID WP_004182757.1
Coordinates 718912..719214 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWB63_RS03420 714064..714843 - 780 WP_052124412.1 hypothetical protein -
AWB63_RS03425 715165..715746 - 582 Protein_643 HNH endonuclease -
AWB63_RS03430 715758..718748 - 2991 WP_038675717.1 type I restriction endonuclease subunit R -
AWB63_RS03435 718912..719214 - 303 WP_004182757.1 helix-turn-helix transcriptional regulator Antitoxin
AWB63_RS03440 719216..719569 - 354 WP_004182758.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AWB63_RS03445 719806..720570 - 765 WP_038675716.1 SDR family NAD(P)-dependent oxidoreductase -
AWB63_RS03450 720602..721759 - 1158 WP_038675715.1 DUF2974 domain-containing protein -
AWB63_RS03455 721869..722495 + 627 WP_002890606.1 Pr6Pr family membrane protein -
AWB63_RS03460 722533..723375 - 843 WP_038675714.1 Cof-type HAD-IIB family hydrolase -
AWB63_RS03465 723527..723916 + 390 WP_038675713.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(35-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-91)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 14074.47 Da        Isoelectric Point: 10.3788

>T60261 WP_004182758.1 NZ_CP014144:c719569-719216 [Streptococcus salivarius]
MYPIHFYKDRNGHEPVREYIETLSMNSSKDSRIKLHKIQDYLTMLKNRGTMIGEPFIKHLEGEIWELRPLRDRILFAAWL
DDGFILLHHFVKKTQKTPRRELMKAQKALDDIRKRGL

Download         Length: 354 bp

>T60261 NZ_CP014144:c719569-719216 [Streptococcus salivarius]
ATGTATCCTATTCATTTTTATAAAGATAGAAATGGACATGAACCTGTTAGAGAGTATATTGAAACATTATCTATGAATTC
AAGTAAAGATTCTAGAATTAAATTACATAAAATTCAAGACTACCTGACTATGTTAAAAAATAGAGGAACCATGATTGGTG
AACCATTCATTAAACATTTGGAAGGAGAGATTTGGGAACTAAGACCCTTAAGAGATAGAATTTTATTTGCAGCATGGCTA
GATGATGGTTTTATTTTGCTTCATCATTTTGTGAAAAAGACACAAAAGACTCCAAGACGTGAGCTGATGAAGGCTCAAAA
GGCTTTAGATGATATTAGAAAGCGAGGATTGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 10936.85 Da        Isoelectric Point: 9.7589

>AT60261 WP_004182757.1 NZ_CP014144:c719214-718912 [Streptococcus salivarius]
MTPEKNPAIGSSWDDLQKTIFTKEEIAASELRVALMLEIIDARKKQGISQKKLEELSGVKQPVIARMETGKTAPQLDTVL
KILASLGKTLAVVPLDSHVK

Download         Length: 303 bp

>AT60261 NZ_CP014144:c719214-718912 [Streptococcus salivarius]
ATGACTCCAGAAAAGAATCCAGCAATTGGAAGTAGTTGGGATGATTTACAGAAAACTATTTTCACTAAAGAGGAAATCGC
GGCAAGTGAACTCCGTGTCGCACTGATGCTTGAGATTATTGATGCTCGTAAAAAACAAGGCATTTCTCAAAAGAAACTTG
AAGAATTGAGTGGTGTCAAACAACCTGTTATCGCACGTATGGAGACAGGTAAAACGGCACCTCAGTTAGATACGGTTCTT
AAAATACTGGCTAGTCTAGGTAAGACATTGGCAGTAGTCCCACTTGATTCACATGTGAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S4MPX5

Antitoxin

Source ID Structure
AlphaFold DB A0A448A9W2

References