Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5339613..5340175 Replicon chromosome
Accession NZ_CP014137
Organism Brenneria goodwinii strain FRB141

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag AWC36_RS23800 Protein ID WP_095835669.1
Coordinates 5339897..5340175 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag AWC36_RS23795 Protein ID WP_095835668.1
Coordinates 5339613..5339897 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWC36_RS23775 5335934..5336899 + 966 WP_095835664.1 toprim domain-containing protein -
AWC36_RS23780 5336896..5337906 + 1011 WP_095835665.1 DUF968 domain-containing protein -
AWC36_RS23785 5337899..5338294 + 396 WP_095835666.1 antitermination protein Q -
AWC36_RS23790 5338538..5339590 + 1053 WP_095835667.1 site-specific DNA-methyltransferase -
AWC36_RS23795 5339613..5339897 - 285 WP_095835668.1 HigA family addiction module antidote protein Antitoxin
AWC36_RS23800 5339897..5340175 - 279 WP_095835669.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AWC36_RS23805 5340333..5340587 + 255 WP_095835670.1 phage holin family protein -
AWC36_RS23810 5340584..5341141 + 558 WP_095835671.1 lysozyme -
AWC36_RS23815 5341120..5341548 + 429 WP_095835672.1 lysis protein -
AWC36_RS23820 5341603..5342040 - 438 WP_095835673.1 hypothetical protein -
AWC36_RS23825 5342100..5342441 + 342 WP_095835674.1 HNH endonuclease -
AWC36_RS23830 5342587..5343042 + 456 WP_095835675.1 phage terminase small subunit P27 family -
AWC36_RS23835 5343045..5344763 + 1719 WP_095835676.1 terminase large subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(10-67)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10477.89 Da        Isoelectric Point: 9.4011

>T60255 WP_095835669.1 NZ_CP014137:c5340175-5339897 [Brenneria goodwinii]
MIKSFKHKGLRQFFEKGSTAGIDAKQAGKIRLRLAVIDAAEEIKDIDRPGYSLHSLTGDKKGQWSITVTGNWRITFEFID
GNAYIVNYEDYH

Download         Length: 279 bp

>T60255 NZ_CP014137:c5340175-5339897 [Brenneria goodwinii]
ATGATTAAAAGCTTCAAGCACAAAGGGTTGCGTCAGTTCTTCGAAAAAGGCTCCACCGCAGGAATAGATGCAAAACAAGC
TGGGAAAATCAGGCTTCGTCTCGCGGTTATTGATGCAGCGGAAGAGATAAAGGATATTGACCGCCCTGGCTACAGTCTCC
ACTCGTTAACCGGAGACAAAAAAGGACAATGGTCCATAACCGTTACAGGGAACTGGCGCATTACATTTGAATTTATCGAT
GGGAATGCCTACATCGTTAATTATGAGGATTACCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10072.59 Da        Isoelectric Point: 6.4760

>AT60255 WP_095835668.1 NZ_CP014137:c5339897-5339613 [Brenneria goodwinii]
MTMHNPPHPGEIIAETLEELGVSIRELARALGVAPSTVQRLVAGNAAISPEMAVKLSTVLGSSAKFWLNLQDNYSLWVAQ
HTVDTSTLHKLVAA

Download         Length: 285 bp

>AT60255 NZ_CP014137:c5339897-5339613 [Brenneria goodwinii]
ATGACTATGCATAACCCCCCGCATCCGGGGGAAATCATTGCCGAAACCCTAGAAGAATTGGGCGTAAGCATCAGAGAACT
AGCCAGGGCACTCGGTGTAGCGCCTTCCACTGTACAACGTCTTGTTGCGGGCAATGCTGCTATCAGTCCTGAAATGGCAG
TAAAGCTGTCTACTGTTCTGGGTAGTTCGGCGAAGTTTTGGCTAAACCTTCAGGACAATTACAGTCTATGGGTAGCTCAG
CATACCGTTGACACGTCAACCTTGCACAAACTTGTTGCCGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References