Detailed information of TA system TA060218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 391777..392434
• Replicon: plasmid unnamed2
• Accession: NZ_CP014127
• Organism: Pantoea vagans strain FDAARGOS_160

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AL522_RS02345
• Protein ID: WP_061060827.1
• Coordinates: 391777..392148 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5M7LSY0
• Locus tag: AL522_RS02350
• Protein ID: WP_061060828.1
• Coordinates: 392135..392434 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL522_RS02335	388217..389725	+	1509	WP_013196201.1	anion permease	-
AL522_RS02340	389882..391528	+	1647	WP_061060826.1	fumarate hydratase	-
AL522_RS02345	391777..392148	+	372	WP_061060827.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AL522_RS02350	392135..392434	+	300	WP_061060828.1	XRE family transcriptional regulator	Antitoxin
AL522_RS02355	392610..393266	-	657	WP_061060829.1	SDR family oxidoreductase	-
AL522_RS02360	393356..394423	-	1068	WP_061060830.1	SDR family oxidoreductase	-
AL522_RS02365	394524..395426	+	903	WP_061060831.1	LysR family transcriptional regulator	-
AL522_RS02370	395748..397158	+	1411	Protein_365	sugar porter family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla / icmF/tssM / tssF / iroN	1..470309	470309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14301.27 Da
• Isoelectric Point: 4.7254

>T60218 WP_061060827.1 NZ_CP014127:391777-392148 [Pantoea vagans]
MTWEVITRTLFDVWYEKQTDDVQEEMAAYFLILEEIGPQLGRPHVDQVKASEYKNMKELRVQIGGHPFRAFFAFDPERKA
IVLCAGDKKGEDEKLFYKRMIKIADAEFTSHLESPEIKDDGNA

Nucleotide

• Length: 372 bp

>T60218 NZ_CP014127:391777-392148 [Pantoea vagans]
ATGACATGGGAAGTCATCACAAGAACGTTGTTTGATGTCTGGTATGAAAAGCAGACCGACGATGTTCAGGAAGAAATGGC
AGCCTACTTCCTGATCCTAGAAGAGATTGGCCCCCAATTAGGGCGTCCGCATGTAGATCAGGTTAAAGCGTCTGAATATA
AAAACATGAAAGAGTTGCGTGTACAGATTGGCGGACATCCATTTCGCGCATTCTTCGCCTTTGATCCTGAACGCAAGGCG
ATTGTCCTGTGTGCAGGAGATAAAAAAGGTGAAGATGAAAAACTGTTCTACAAACGTATGATTAAAATTGCAGATGCTGA
GTTCACCTCGCATCTGGAATCACCGGAGATTAAAGACGATGGCAACGCTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10952.69 Da
• Isoelectric Point: 9.8592

>AT60218 WP_061060828.1 NZ_CP014127:392135-392434 [Pantoea vagans]
MATLKEMMAKRSPESRARIESRAAEIRQEITLARLREELSVSQTHLAEVLGVKQPSVAKIENVDNDPKLSTLRRYIAGLG
GELTLDVTLPDGKRIALSL

Nucleotide

• Length: 300 bp

>AT60218 NZ_CP014127:392135-392434 [Pantoea vagans]
ATGGCAACGCTTAAAGAAATGATGGCTAAACGCTCCCCGGAGAGCCGTGCGCGTATAGAATCGCGTGCTGCTGAAATCCG
TCAGGAGATCACACTTGCCAGACTGCGTGAGGAATTATCTGTTTCGCAGACACATTTGGCTGAAGTATTAGGCGTCAAAC
AACCTTCTGTTGCTAAAATAGAAAACGTTGATAACGATCCTAAGCTCTCAACGCTGAGACGCTATATTGCCGGTCTGGGA
GGAGAGCTGACTCTTGATGTCACGTTACCCGACGGCAAGCGTATTGCTCTGAGTCTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A5M7LSY0