Detailed information of TA system TA060180

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 63965..64378
• Replicon: plasmid unnamed2
• Accession: NZ_CP014112
• Organism: Escherichia coli strain FDAARGOS_144

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: AL502_RS26025
• Protein ID: WP_000775238.1
• Coordinates: 63965..64126 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	64178..64378 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL502_RS25965	59396..59998	+	603	WP_077774972.1	transglycosylase SLT domain-containing protein	-
AL502_RS25970	60295..61116	-	822	WP_001234445.1	DUF945 domain-containing protein	-
AL502_RS25975	61226..61522	-	297	WP_001272251.1	hypothetical protein	-
AL502_RS26000	62495..62737	-	243	WP_000540588.1	hypothetical protein	-
AL502_RS26005	62796..63119	-	324	WP_000533253.1	hypothetical protein	-
AL502_RS26020	63415..63727	+	313	Protein_79	hypothetical protein	-
AL502_RS26025	63965..64126	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	64178..64378	-	201	NuclAT_0	-	Antitoxin
-	64178..64378	-	201	NuclAT_0	-	Antitoxin
-	64178..64378	-	201	NuclAT_0	-	Antitoxin
-	64178..64378	-	201	NuclAT_0	-	Antitoxin
AL502_RS26035	64335..64649	-	315	WP_000870669.1	hypothetical protein	-
AL502_RS26040	64646..65365	-	720	WP_020246013.1	plasmid SOS inhibition protein A	-
AL502_RS26045	65362..65796	-	435	WP_000845950.1	conjugation system SOS inhibitor PsiB	-
AL502_RS26050	65851..67800	-	1950	WP_000109422.1	ParB/RepB/Spo0J family partition protein	-
AL502_RS26055	67861..68094	-	234	WP_000005971.1	DUF905 domain-containing protein	-
AL502_RS26060	68150..68671	-	522	WP_000290849.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..69183	69183

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T60180 WP_000775238.1 NZ_CP014112:c64126-63965 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T60180 NZ_CP014112:c64126-63965 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 201 bp

>AT60180 NZ_CP014112:c64378-64178 [Escherichia coli]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATGAGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAAAGCCCCGT
AGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCTA

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin