Detailed information of TA system TA060178

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 21613..21866
• Replicon: plasmid unnamed2
• Accession: NZ_CP014112
• Organism: Escherichia coli strain FDAARGOS_144

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: AL502_RS25730
• Protein ID: WP_001336447.1
• Coordinates: 21613..21762 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	21810..21866 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL502_RS25680	17588..18253	+	666	WP_001535733.1	hypothetical protein	-
AL502_RS25685	18289..18588	-	300	WP_001528606.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AL502_RS25690	18578..18841	-	264	WP_001089473.1	hypothetical protein	-
AL502_RS25695	18948..19208	-	261	WP_024187481.1	hypothetical protein	-
AL502_RS25710	19911..20768	-	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
AL502_RS25715	20761..20835	-	75	WP_001365705.1	RepA leader peptide Tap	-
AL502_RS25725	21071..21328	-	258	WP_000084404.1	replication regulatory protein RepA	-
AL502_RS25730	21613..21762	-	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	21810..21866	+	57	NuclAT_1	-	Antitoxin
-	21810..21866	+	57	NuclAT_1	-	Antitoxin
-	21810..21866	+	57	NuclAT_1	-	Antitoxin
-	21810..21866	+	57	NuclAT_1	-	Antitoxin
AL502_RS25740	21961..22398	-	438	WP_000872609.1	hypothetical protein	-
AL502_RS26580	22551..22935	-	385	Protein_31	hypothetical protein	-
AL502_RS25750	23038..23499	-	462	WP_000760080.1	thermonuclease family protein	-
AL502_RS25755	24241..24444	-	204	WP_001336517.1	hypothetical protein	-
AL502_RS25760	24596..25153	-	558	WP_000139329.1	fertility inhibition protein FinO	-
AL502_RS25765	25208..25954	-	747	WP_032153586.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..69183	69183

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T60178 WP_001336447.1 NZ_CP014112:c21762-21613 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T60178 NZ_CP014112:c21762-21613 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT60178 NZ_CP014112:21810-21866 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin