Detailed information of TA system TA060175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4412862..4413556
• Replicon: chromosome
• Accession: NZ_CP014111
• Organism: Escherichia coli strain FDAARGOS_144

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: AL502_RS23335
• Protein ID: WP_001263500.1
• Coordinates: 4413158..4413556 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AL502_RS23330
• Protein ID: WP_000554758.1
• Coordinates: 4412862..4413155 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL502_RS23310	4408602..4409099	+	498	WP_000006239.1	REP-associated tyrosine transposase RayT	-
AL502_RS23315	4409215..4410927	-	1713	Protein_4143	flagellar biosynthesis protein FlhA	-
AL502_RS23320	4410899..4411684	+	786	WP_000207551.1	putative lateral flagellar export/assembly protein LafU	-
AL502_RS23325	4411755..4412810	+	1056	WP_001226168.1	DNA polymerase IV	-
AL502_RS23330	4412862..4413155	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AL502_RS23335	4413158..4413556	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AL502_RS23340	4413566..4414018	+	453	WP_071589393.1	GNAT family N-acetyltransferase	-
AL502_RS23345	4414208..4415347	+	1140	WP_022296578.1	RNA ligase RtcB family protein	-
AL502_RS23350	4415344..4415958	+	615	WP_059346544.1	peptide chain release factor H	-
AL502_RS23355	4416014..4417471	-	1458	WP_001293022.1	cytosol nonspecific dipeptidase	-
AL502_RS23360	4417732..4418190	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T60175 WP_001263500.1 NZ_CP014111:4413158-4413556 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T60175 NZ_CP014111:4413158-4413556 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT60175 WP_000554758.1 NZ_CP014111:4412862-4413155 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT60175 NZ_CP014111:4412862-4413155 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3