Detailed information of TA system TA060168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3500516..3501118
• Replicon: chromosome
• Accession: NZ_CP014111
• Organism: Escherichia coli strain FDAARGOS_144

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3L4YH82
• Locus tag: AL502_RS18935
• Protein ID: WP_000897303.1
• Coordinates: 3500516..3500827 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AL502_RS18940
• Protein ID: WP_000356397.1
• Coordinates: 3500828..3501118 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL502_RS18910	3496430..3497029	+	600	WP_001296610.1	glucose-1-phosphatase	-
AL502_RS18915	3497023..3497895	+	873	WP_000920763.1	virulence factor BrkB family protein	-
AL502_RS18920	3497892..3498329	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AL502_RS18925	3498374..3499315	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AL502_RS18930	3499379..3500287	-	909	WP_001385591.1	alpha/beta hydrolase	-
AL502_RS18935	3500516..3500827	+	312	WP_000897303.1	hypothetical protein	Toxin
AL502_RS18940	3500828..3501118	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AL502_RS18950	3501477..3501755	+	279	WP_001296612.1	hypothetical protein	-
AL502_RS18960	3502154..3502372	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
AL502_RS18965	3502557..3503006	-	450	WP_033555719.1	hypothetical protein	-
AL502_RS18970	3503322..3504170	-	849	WP_001038651.1	hypothetical protein	-
AL502_RS18975	3504460..3504702	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
AL502_RS18980	3504884..3505813	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12302.33 Da
• Isoelectric Point: 10.0233

>T60168 WP_000897303.1 NZ_CP014111:3500516-3500827 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGRLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T60168 NZ_CP014111:3500516-3500827 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTAGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAGGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT60168 WP_000356397.1 NZ_CP014111:3500828-3501118 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT60168 NZ_CP014111:3500828-3501118 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A3L4YH82