Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2684457..2685256 Replicon chromosome
Accession NZ_CP014111
Organism Escherichia coli strain FDAARGOS_144

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag AL502_RS14745 Protein ID WP_000347251.1
Coordinates 2684792..2685256 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag AL502_RS14740 Protein ID WP_001296435.1
Coordinates 2684457..2684792 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL502_RS14725 2680242..2681012 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
AL502_RS14730 2681028..2682362 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
AL502_RS14735 2682737..2684308 + 1572 WP_001273763.1 galactarate dehydratase -
AL502_RS14740 2684457..2684792 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
AL502_RS14745 2684792..2685256 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
AL502_RS14750 2685314..2686120 - 807 WP_022296693.1 aga operon transcriptional regulator AgaR -
AL502_RS14760 2686369..2687649 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
AL502_RS14765 2687672..2688145 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
AL502_RS14770 2688156..2688935 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
AL502_RS14775 2688925..2689803 + 879 WP_001298314.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
AL502_RS14780 2689821..2690255 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T60162 WP_000347251.1 NZ_CP014111:2684792-2685256 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T60162 NZ_CP014111:2684792-2685256 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT60162 WP_001296435.1 NZ_CP014111:2684457-2684792 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT60162 NZ_CP014111:2684457-2684792 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References