Detailed information of TA system TA060159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1398333..1398835
• Replicon: chromosome
• Accession: NZ_CP014111
• Organism: Escherichia coli strain FDAARGOS_144

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: AL502_RS08365
• Protein ID: WP_000767819.1
• Coordinates: 1398333..1398587 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: AL502_RS08370
• Protein ID: WP_001259253.1
• Coordinates: 1398584..1398835 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL502_RS08335	1394151..1394609	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
AL502_RS08345	1394826..1396184	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
AL502_RS08350	1396174..1396236	-	63	WP_010723108.1	membrane protein YoeI	-
AL502_RS08355	1396451..1397380	-	930	WP_000803366.1	LysR family transcriptional regulator	-
AL502_RS08360	1397426..1398250	-	825	WP_000754733.1	SDR family oxidoreductase	-
AL502_RS08365	1398333..1398587	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
AL502_RS08370	1398584..1398835	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
AL502_RS08380	1399118..1399168	+	51	WP_001364200.1	his operon leader peptide	-
AL502_RS08390	1399314..1400213	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
AL502_RS08395	1400219..1401523	+	1305	WP_001524971.1	histidinol dehydrogenase	-
AL502_RS08400	1401520..1402590	+	1071	WP_001524973.1	histidinol-phosphate transaminase	-
AL502_RS08405	1402590..1403657	+	1068	WP_000080128.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1394151..1394609	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T60159 WP_000767819.1 NZ_CP014111:c1398587-1398333 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T60159 NZ_CP014111:c1398587-1398333 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT60159 WP_001259253.1 NZ_CP014111:c1398835-1398584 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT60159 NZ_CP014111:c1398835-1398584 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3