Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 806233..806795 Replicon chromosome
Accession NZ_CP014111
Organism Escherichia coli strain FDAARGOS_144

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag AL502_RS05045 Protein ID WP_000605675.1
Coordinates 806517..806795 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AL502_RS05040 Protein ID WP_000781370.1
Coordinates 806233..806517 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL502_RS05025 801415..804462 + 3048 WP_141095332.1 formate dehydrogenase-N subunit alpha -
AL502_RS05030 804475..805359 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
AL502_RS05035 805352..806005 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
AL502_RS05040 806233..806517 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
AL502_RS05045 806517..806795 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AL502_RS05050 806981..807991 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
AL502_RS05055 808125..809822 - 1698 WP_001607334.1 malate dehydrogenase -
AL502_RS05060 809978..810115 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AL502_RS05065 810217..810432 - 216 WP_000495774.1 biofilm-dependent modulation protein -
AL502_RS05075 810776..811207 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T60148 WP_000605675.1 NZ_CP014111:c806795-806517 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T60148 NZ_CP014111:c806795-806517 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT60148 WP_000781370.1 NZ_CP014111:c806517-806233 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT60148 NZ_CP014111:c806517-806233 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References