Detailed information of TA system TA060142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4720922..4721649
• Replicon: chromosome
• Accession: NZ_CP014099
• Organism: Shigella sonnei strain FDAARGOS_90

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: AL477_RS24715
• Protein ID: WP_000547563.1
• Coordinates: 4720922..4721233 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AL477_RS24720
• Protein ID: WP_000126296.1
• Coordinates: 4721230..4721649 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL477_RS24685 (4716076)	4716076..4717773	+	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-
AL477_RS24690 (4717783)	4717783..4718325	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
AL477_RS24695 (4718325)	4718325..4719092	+	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
AL477_RS24700 (4719089)	4719089..4719499	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
AL477_RS24705 (4719492)	4719492..4719962	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
AL477_RS24710 (4719987)	4719987..4720760	+	774	WP_001026459.1	hypothetical protein	-
AL477_RS24715 (4720922)	4720922..4721233	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
AL477_RS24720 (4721230)	4721230..4721649	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
AL477_RS24725 (4721763)	4721763..4723187	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
AL477_RS24730 (4723196)	4723196..4724653	-	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
AL477_RS24735 (4724913)	4724913..4725923	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
AL477_RS24740 (4726071)	4726071..4726598	+	528	WP_001078766.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T60142 WP_000547563.1 NZ_CP014099:4720922-4721233 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T60142 NZ_CP014099:4720922-4721233 [Shigella sonnei]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT60142 WP_000126296.1 NZ_CP014099:4721230-4721649 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT60142 NZ_CP014099:4721230-4721649 [Shigella sonnei]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22