Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2553748..2554289 Replicon chromosome
Accession NZ_CP014099
Organism Shigella sonnei strain FDAARGOS_90

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag AL477_RS13890 Protein ID WP_000615976.1
Coordinates 2554011..2554289 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AL477_RS13885 Protein ID WP_000729704.1
Coordinates 2553748..2554008 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL477_RS13860 (2549217) 2549217..2550929 + 1713 Protein_2555 flagellar biosynthesis protein FlhA -
AL477_RS13865 (2551052) 2551052..2551549 - 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
AL477_RS13870 (2551620) 2551620..2551748 - 129 Protein_2557 ISAs1 family transposase -
AL477_RS13875 (2551803) 2551803..2552500 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
AL477_RS13880 (2552789) 2552789..2553562 - 774 WP_127148796.1 C40 family peptidase -
AL477_RS13885 (2553748) 2553748..2554008 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AL477_RS13890 (2554011) 2554011..2554289 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AL477_RS13895 (2554445) 2554445..2555185 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AL477_RS13900 (2555156) 2555156..2555923 - 768 WP_000333380.1 class II glutamine amidotransferase -
AL477_RS13905 (2556129) 2556129..2556707 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 2552176..2565150 12974


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T60137 WP_000615976.1 NZ_CP014099:2554011-2554289 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T60137 NZ_CP014099:2554011-2554289 [Shigella sonnei]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT60137 WP_000729704.1 NZ_CP014099:2553748-2554008 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT60137 NZ_CP014099:2553748-2554008 [Shigella sonnei]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCTGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References