Detailed information of TA system TA060088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1121725..1122541
• Replicon: chromosome
• Accession: NZ_CP014070
• Organism: Citrobacter amalonaticus strain FDAARGOS_165

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: AL524_RS30985
• Protein ID: WP_061069544.1
• Coordinates: 1122065..1122541 (+)
• Length: 159 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A3Q8DNJ3
• Locus tag: AL524_RS30980
• Protein ID: WP_042999108.1
• Coordinates: 1121725..1122060 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL524_RS30960	1118944..1119570	+	627	WP_042999104.1	dimethylsulfoxide reductase subunit B	-
AL524_RS30965	1119563..1120336	+	774	WP_044257303.1	dimethyl sulfoxide reductase anchor subunit	-
AL524_RS30970	1120381..1120977	+	597	WP_042999106.1	molecular chaperone	-
AL524_RS30975	1120974..1121510	+	537	WP_042999107.1	ferredoxin-type protein NapF	-
AL524_RS30980	1121725..1122060	+	336	WP_042999108.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
AL524_RS30985	1122065..1122541	+	477	WP_061069544.1	type II toxin-antitoxin system YhaV family toxin	Toxin
AL524_RS30990	1122965..1123495	+	531	WP_042999110.1	type 1 fimbrial protein	-
AL524_RS30995	1123663..1124112	+	450	WP_099433589.1	type 1 fimbrial protein	-
AL524_RS31000	1124176..1126650	+	2475	WP_044257386.1	fimbria/pilus outer membrane usher protein	-
AL524_RS31005	1126693..1127436	+	744	WP_044264050.1	molecular chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (15-110)

Sequences

Toxin

Protein

• Length: 159 a.a.
• Molecular weight: 18194.81 Da
• Isoelectric Point: 8.4404

>T60088 WP_061069544.1 NZ_CP014070:1122065-1122541 [Citrobacter amalonaticus]
MDFPTWLNGWTIYAHPCFIDQYNALVARVEDLKHRFPDPIVFQKKKETKVLAHLLKSIANITHEPRASVYLPGDSIGKAY
TDWSRAKFGGGRYRLFFRYSLESKIIVIAWVNDEGPLRTYGSKTDAYKIFGKMLDEGNPPDDWLSLLQACQNDGKEHL

Nucleotide

• Length: 477 bp

>T60088 NZ_CP014070:1122065-1122541 [Citrobacter amalonaticus]
ATGGATTTTCCCACATGGCTGAACGGGTGGACGATCTACGCTCATCCCTGTTTTATCGATCAATACAACGCGCTGGTCGC
ACGTGTTGAAGACCTTAAGCACAGGTTTCCAGATCCCATTGTTTTCCAGAAGAAGAAAGAGACCAAGGTGCTGGCGCATT
TATTGAAATCCATTGCCAACATTACCCACGAACCCCGAGCTTCTGTTTACCTGCCTGGTGACAGTATAGGGAAAGCTTAT
ACAGACTGGTCGAGAGCGAAGTTTGGTGGGGGACGGTATCGCTTATTTTTCCGTTATAGTCTCGAGAGTAAGATCATTGT
CATCGCATGGGTGAATGATGAAGGGCCATTGCGCACCTACGGCAGCAAGACCGATGCCTATAAGATTTTCGGGAAAATGC
TCGATGAGGGGAATCCACCCGACGACTGGCTAAGTTTGCTTCAGGCTTGTCAAAACGACGGGAAGGAACATCTTTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12505.83 Da
• Isoelectric Point: 4.1876

>AT60088 WP_042999108.1 NZ_CP014070:1121725-1122060 [Citrobacter amalonaticus]
MFAYVRSEDIAIEADSKLTERHQTTIPASIRDALHLRSGDRIHYTLLSTGEVLLSKYSEATEDGVMRSFLDFLSQDVANN
PQTLKKLDLSRGYELVDGIDVNLDDEISDED

Nucleotide

• Length: 336 bp

>AT60088 NZ_CP014070:1121725-1122060 [Citrobacter amalonaticus]
ATGTTCGCCTATGTTCGATCTGAAGACATTGCCATTGAAGCGGATTCTAAATTGACGGAACGACATCAAACGACTATCCC
TGCGTCGATCAGGGACGCTTTACATCTTCGCAGTGGTGATCGTATTCATTACACATTGCTTTCCACGGGTGAAGTACTGC
TTTCAAAATACAGTGAAGCAACGGAGGACGGTGTGATGCGATCATTCCTGGACTTCTTATCACAGGATGTGGCGAATAAC
CCACAAACGCTGAAGAAGCTGGATTTATCCCGTGGCTATGAACTGGTTGATGGTATCGACGTCAATCTTGATGATGAAAT
CAGCGACGAAGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A3Q8DNJ3