Detailed information of TA system TA060057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE(toxin)
• Location: 6014088..6014721
• Replicon: chromosome
• Accession: NZ_CP014060
• Organism: Achromobacter xylosoxidans strain FDAARGOS_147

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A120LI58
• Locus tag: AL504_RS27580
• Protein ID: WP_061073800.1
• Coordinates: 6014088..6014399 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AL504_RS27585
• Protein ID: WP_192579613.1
• Coordinates: 6014449..6014721 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL504_RS27550	6009566..6010456	-	891	WP_061074524.1	alpha/beta hydrolase	-
AL504_RS27555	6010597..6011184	+	588	WP_061073798.1	CGNR zinc finger domain-containing protein	-
AL504_RS27560	6011188..6011589	-	402	WP_061074525.1	hypothetical protein	-
AL504_RS27565	6011717..6012334	-	618	WP_104021734.1	LysE family translocator	-
AL504_RS27570	6012416..6012868	+	453	WP_104021735.1	Lrp/AsnC family transcriptional regulator	-
AL504_RS27575	6012879..6013814	-	936	WP_061073799.1	hypothetical protein	-
AL504_RS27580	6014088..6014399	+	312	WP_061073800.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AL504_RS27585	6014449..6014721	+	273	WP_192579613.1	helix-turn-helix domain-containing protein	Antitoxin
AL504_RS27590	6014756..6015373	-	618	WP_061073802.1	LysE family transporter	-
AL504_RS27595	6015520..6017211	-	1692	WP_061073803.1	hypothetical protein	-
AL504_RS27600	6017566..6018273	-	708	WP_061073804.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (40-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-87)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11597.42 Da
• Isoelectric Point: 9.6219

>T60057 WP_061073800.1 NZ_CP014060:6014088-6014399 [Achromobacter xylosoxidans]
MEFVETPLFTKQITALLSDDEYRGLQMLLVENPERGDLVKGGGGIRKIRYARPGTGKSGGLRAIYYWISRDDTVYMLVAY
PKSRKDGLTREETAILAAKVKEL

Nucleotide

• Length: 312 bp

>T60057 NZ_CP014060:6014088-6014399 [Achromobacter xylosoxidans]
ATGGAATTTGTAGAAACGCCGCTGTTCACCAAACAGATCACCGCGCTGCTATCCGACGATGAGTACCGTGGATTGCAGAT
GCTCCTCGTAGAGAATCCAGAGCGCGGTGATCTCGTCAAGGGCGGGGGCGGCATTCGCAAGATCCGCTATGCACGCCCGG
GCACGGGAAAAAGCGGTGGTTTGCGCGCGATCTATTACTGGATCTCTCGCGACGACACGGTCTACATGCTTGTCGCGTAT
CCCAAGTCACGCAAGGACGGCCTTACGCGCGAAGAAACCGCGATTCTGGCCGCCAAAGTGAAGGAGCTATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9993.54 Da
• Isoelectric Point: 11.6528

>AT60057 WP_192579613.1 NZ_CP014060:6014449-6014721 [Achromobacter xylosoxidans]
MLNEGLDQARAIRRGGLAPGRVTKFEPIDVRAIRERVQMSQAEFAAAIHVSTRTLQNWEQRHRNPTGPAIALLKIVERAP
DVAVKVLQSA

Nucleotide

• Length: 273 bp

>AT60057 NZ_CP014060:6014449-6014721 [Achromobacter xylosoxidans]
CTGTTGAACGAAGGGTTGGACCAGGCGCGAGCCATTCGCAGGGGGGGACTGGCGCCCGGCCGGGTCACGAAGTTCGAGCC
GATCGACGTGCGGGCCATTCGTGAGCGTGTCCAGATGTCCCAGGCCGAATTCGCCGCGGCCATCCATGTCAGCACCCGCA
CGCTGCAGAATTGGGAGCAGCGGCACCGAAATCCAACCGGGCCTGCCATCGCGCTGCTCAAGATCGTGGAACGCGCGCCC
GACGTCGCCGTGAAAGTGCTTCAATCCGCATGA

Structures

Toxin

• AlphaFold DB: A0A120LI58