Detailed information of TA system TA060038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 165025..165670
• Replicon: plasmid unnamed1
• Accession: NZ_CP014052
• Organism: Vibrio alginolyticus strain FDAARGOS_108

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2U3B7P5
• Locus tag: AL539_RS00890
• Protein ID: WP_041156865.1
• Coordinates: 165025..165375 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AL539_RS00895
• Protein ID: WP_041156866.1
• Coordinates: 165365..165670 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL539_RS00870	161136..161639	-	504	WP_041156862.1	hypothetical protein	-
AL539_RS00875	161889..162383	-	495	WP_041156863.1	TIGR00645 family protein	-
AL539_RS00880	162977..163999	-	1023	WP_102552417.1	IS110 family transposase	-
AL539_RS00885	164136..164762	-	627	WP_041156864.1	recombinase family protein	-
AL539_RS00890	165025..165375	+	351	WP_041156865.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AL539_RS00895	165365..165670	+	306	WP_041156866.1	XRE family transcriptional regulator	Antitoxin
AL539_RS00900	166052..166270	-	219	WP_104975176.1	hypothetical protein	-
AL539_RS00905	166694..167134	-	441	WP_104975177.1	hypothetical protein	-
AL539_RS00910	167602..168003	+	402	WP_104975178.1	DUF1413 domain-containing protein	-
AL539_RS00915	168246..168539	-	294	WP_104975179.1	hypothetical protein	-
AL539_RS00920	168619..170045	-	1427	Protein_177	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..217123	217123

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-90)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13481.40 Da
• Isoelectric Point: 4.9948

>T60038 WP_041156865.1 NZ_CP014052:165025-165375 [Vibrio alginolyticus]
MWNVVTRPLFDEWFLSLDESDQINVRACIQVLLERGPQLSRPYADTVEDSKHSNMKELRVQINGKPIRAFFAFDPERTGI
ILCGGEKTGDKRFYKKMIPIADAELDEHLEELKNEK

Nucleotide

• Length: 351 bp

>T60038 NZ_CP014052:165025-165375 [Vibrio alginolyticus]
ATGTGGAATGTAGTAACTAGACCACTTTTTGACGAGTGGTTTCTATCACTAGATGAAAGCGATCAAATTAATGTCCGCGC
ATGCATCCAGGTTCTTCTTGAAAGAGGGCCTCAGCTGAGCAGACCATACGCTGACACTGTGGAAGATTCAAAGCACAGCA
ACATGAAAGAGTTGCGAGTGCAAATCAATGGGAAACCTATACGAGCTTTCTTTGCTTTTGACCCAGAAAGAACAGGAATT
ATCTTATGTGGCGGTGAAAAAACTGGCGATAAGAGATTCTACAAAAAGATGATTCCGATTGCTGATGCAGAACTTGATGA
GCATTTAGAGGAACTTAAAAATGAAAAGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10972.82 Da
• Isoelectric Point: 8.5752

>AT60038 WP_041156866.1 NZ_CP014052:165365-165670 [Vibrio alginolyticus]
MKSNPLSVLDAKMSPEALAAAKEKAADMLLDIRLAELREITHLSQVEIAKAMGISQPSVANLEKSGKDLRLSSLKKYIEA
TGGKLRIDVELPDGKHFSFPV

Nucleotide

• Length: 306 bp

>AT60038 NZ_CP014052:165365-165670 [Vibrio alginolyticus]
ATGAAAAGTAACCCATTAAGCGTACTTGACGCAAAAATGTCACCAGAAGCACTTGCTGCCGCTAAAGAAAAAGCAGCGGA
TATGCTTCTTGATATTCGACTTGCTGAGCTTCGAGAGATCACCCATCTATCTCAAGTAGAAATCGCTAAAGCTATGGGCA
TTAGCCAGCCATCAGTTGCCAATTTAGAGAAATCTGGCAAAGATTTACGTCTTAGCTCTCTAAAGAAATACATCGAAGCG
ACCGGTGGTAAGCTTCGCATTGATGTAGAACTGCCAGACGGAAAACATTTTAGCTTCCCGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A2U3B7P5