Detailed information of TA system TA060011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1174564..1175179
• Replicon: chromosome
• Accession: NZ_CP014042
• Organism: Vibrio mimicus strain FDAARGOS_112

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: AL543_RS05780
• Protein ID: WP_001162670.1
• Coordinates: 1174892..1175179 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AL543_RS05775
• Protein ID: WP_001232701.1
• Coordinates: 1174564..1174881 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL543_RS05720	1169568..1169738	-	171	WP_001080654.1	hypothetical protein	-
AL543_RS05725	1169918..1170301	-	384	WP_061051356.1	hypothetical protein	-
AL543_RS05730	1170452..1170838	-	387	WP_061051357.1	VOC family protein	-
AL543_RS05735	1170828..1170929	-	102	WP_081096080.1	acetyltransferase	-
AL543_RS05740	1171014..1171562	-	549	WP_061051358.1	hypothetical protein	-
AL543_RS05745	1171582..1171704	-	123	Protein_1105	DUF3265 domain-containing protein	-
AL543_RS05750	1171701..1172060	-	360	WP_029856942.1	VOC family protein	-
AL543_RS05755	1172345..1172605	-	261	WP_151898470.1	DUF3709 domain-containing protein	-
AL543_RS05760	1172875..1173426	-	552	WP_061051359.1	sugar O-acetyltransferase	-
AL543_RS05765	1173590..1174429	-	840	WP_055032240.1	hypothetical protein	-
AL543_RS21885	1174457..1174567	-	111	WP_082798255.1	DUF3265 domain-containing protein	-
AL543_RS05775	1174564..1174881	-	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
AL543_RS05780	1174892..1175179	-	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AL543_RS05790	1175409..1175822	-	414	WP_061051312.1	VOC family protein	-
AL543_RS05800	1176107..1176244	-	138	WP_000757220.1	YHYH domain-containing protein	-
AL543_RS05805	1176582..1176815	-	234	WP_081096082.1	DUF3709 domain-containing protein	-
AL543_RS21505	1176836..1176967	-	132	WP_151190709.1	DUF3709 domain-containing protein	-
AL543_RS05810	1177153..1177395	+	243	WP_011080384.1	type II toxin-antitoxin system ParD family antitoxin	-
AL543_RS05815	1177392..1177691	+	300	WP_017420621.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AL543_RS21890	1177699..1177791	-	93	WP_208620269.1	DUF3265 domain-containing protein	-
AL543_RS05825	1177915..1178268	+	354	WP_061051360.1	hypothetical protein	-
AL543_RS21510	1178574..1178762	-	189	Protein_1121	DUF645 family protein	-
AL543_RS05845	1179048..1179491	-	444	WP_061051361.1	hypothetical protein	-
AL543_RS05850	1179774..1179992	-	219	WP_061051362.1	DUF3709 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1086679..1234651	147972
	-	inside	Integron	-	-	1108123..1230683	122560

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T60011 WP_001162670.1 NZ_CP014042:c1175179-1174892 [Vibrio mimicus]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T60011 NZ_CP014042:c1175179-1174892 [Vibrio mimicus]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT60011 WP_001232701.1 NZ_CP014042:c1174881-1174564 [Vibrio mimicus]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT60011 NZ_CP014042:c1174881-1174564 [Vibrio mimicus]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTGAGTAATTTGATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCAGCGGCATTAGGCAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36