Detailed information of TA system TA059969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2629422..2629984
• Replicon: chromosome
• Accession: NZ_CP014030
• Organism: Citrobacter sp. FDAARGOS_156 strain FDAARGOS_155

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AL515_RS12835
• Protein ID: WP_048212587.1
• Coordinates: 2629422..2629700 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AL515_RS12840
• Protein ID: WP_048211649.1
• Coordinates: 2629700..2629984 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL515_RS12815	2624539..2624970	-	432	WP_048211652.1	peroxiredoxin OsmC	-
AL515_RS12820	2625198..2625338	+	141	WP_005126806.1	stationary-phase-induced ribosome-associated protein	-
AL515_RS12825	2625518..2627215	+	1698	WP_048211651.1	malate dehydrogenase	-
AL515_RS12830	2627525..2629240	+	1716	WP_061067906.1	ATP-binding cassette domain-containing protein	-
AL515_RS12835	2629422..2629700	+	279	WP_048212587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AL515_RS12840	2629700..2629984	+	285	WP_048211649.1	HigA family addiction module antidote protein	Antitoxin
AL515_RS12845	2630028..2630630	-	603	WP_061067907.1	inorganic diphosphatase	-
AL515_RS12850	2630759..2631415	-	657	WP_061067908.1	formate dehydrogenase-N subunit gamma	-
AL515_RS12855	2631408..2632292	-	885	WP_003833024.1	formate dehydrogenase N subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10737.31 Da
• Isoelectric Point: 9.9329

>T59969 WP_048212587.1 NZ_CP014030:2629422-2629700 [Citrobacter sp. FDAARGOS_156]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRLAVIDAANKVTDINMPGYQLHPLVGERDGRWAISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T59969 NZ_CP014030:2629422-2629700 [Citrobacter sp. FDAARGOS_156]
ATGATCATGAACTTCAGACACAAGGGATTGCGCGATTTGTTTCTTCATGGACGAACATCAGGCGTTATGGCAAAACATGT
CCGACGATTACGACATAGACTTGCCGTTATTGATGCAGCAAACAAGGTTACAGATATCAATATGCCCGGTTATCAGCTAC
ATCCTTTGGTGGGCGAGCGTGACGGCAGATGGGCAATTTCTGTTTCAGGTAACTGGCGTATTACGTTTGAATTCGTCAAT
GGCGACGCGTACATACTGGACTACGAGGACTATCATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10313.06 Da
• Isoelectric Point: 10.0902

>AT59969 WP_048211649.1 NZ_CP014030:2629700-2629984 [Citrobacter sp. FDAARGOS_156]
MKMANHPRPGDIIQEALDELTVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNAWSLAEAK
KNVDISRLRKLAAQ

Nucleotide

• Length: 285 bp

>AT59969 NZ_CP014030:2629700-2629984 [Citrobacter sp. FDAARGOS_156]
ATGAAGATGGCAAATCATCCCCGTCCTGGGGATATCATTCAGGAAGCGCTGGACGAACTCACTGTCAGCCTGCGCGAGTT
TGCCAGAGCGATGGAGATTGCACCTTCAACGGCAAGTCGGCTATTAACCGGGAAAGCGGCACTAACGCCTGAGATGGCGA
TTAAACTCTCCGTGGTGATTGGCAGTTCACCCGAAATGTGGTTAAACCTGCAAAATGCCTGGAGCCTGGCAGAGGCCAAA
AAAAACGTGGATATTTCACGCCTGCGTAAGCTGGCAGCGCAGTGA