Detailed information of TA system TA059964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 447083..447837
• Replicon: chromosome
• Accession: NZ_CP014030
• Organism: Citrobacter sp. FDAARGOS_156 strain FDAARGOS_155

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AL515_RS02185
• Protein ID: WP_061066951.1
• Coordinates: 447526..447837 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AL515_RS02180
• Protein ID: WP_048214030.1
• Coordinates: 447083..447529 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL515_RS02150	442347..443243	+	897	WP_061066950.1	sugar kinase	-
AL515_RS02155	443277..444080	+	804	WP_003847907.1	DeoR/GlpR family DNA-binding transcription regulator	-
AL515_RS02160	444186..444785	+	600	WP_048214033.1	glucose-1-phosphatase	-
AL515_RS02165	444779..445651	+	873	WP_048214032.1	virulence factor BrkB family protein	-
AL515_RS02170	445648..446085	+	438	WP_003028671.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AL515_RS02175	446130..447071	+	942	WP_048214031.1	fatty acid biosynthesis protein FabY	-
AL515_RS02180	447083..447529	-	447	WP_048214030.1	helix-turn-helix domain-containing protein	Antitoxin
AL515_RS02185	447526..447837	-	312	WP_061066951.1	type II toxin-antitoxin system HigB family toxin	Toxin
AL515_RS02190	447923..448852	-	930	WP_061066952.1	alpha/beta hydrolase	-
AL515_RS02195	449057..449299	+	243	WP_043018956.1	ribbon-helix-helix domain-containing protein	-
AL515_RS02200	449299..449673	+	375	WP_061066953.1	type II toxin-antitoxin system VapC family toxin	-
AL515_RS02205	449710..450639	-	930	WP_061066954.1	formate dehydrogenase accessory protein FdhE	-
AL515_RS02210	450636..451271	-	636	WP_061066955.1	formate dehydrogenase cytochrome b556 subunit	-
AL515_RS02215	451268..452170	-	903	WP_003028691.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12478.48 Da
• Isoelectric Point: 9.4821

>T59964 WP_061066951.1 NZ_CP014030:c447837-447526 [Citrobacter sp. FDAARGOS_156]
VHVISRKPFNEGMLMYPNHGLALSELLNVLEKKTFYHPEEMKQYIPSLDNFKYRNKWWVIDVSGNNLRLIAYIDFRLHKV
FVKHIVLHAEYDKLTEYYRGHKE

Nucleotide

• Length: 312 bp

>T59964 NZ_CP014030:c447837-447526 [Citrobacter sp. FDAARGOS_156]
GTGCATGTTATCAGCCGAAAACCATTCAATGAGGGTATGCTCATGTATCCAAACCATGGGCTTGCTCTCTCTGAGTTGCT
GAATGTTTTGGAGAAAAAAACATTCTATCACCCTGAAGAGATGAAACAGTATATTCCTTCTCTTGATAATTTTAAGTACA
GGAATAAATGGTGGGTAATTGATGTTTCTGGTAACAACCTGAGACTCATTGCCTACATAGATTTCAGGTTACATAAGGTA
TTTGTGAAGCATATCGTTCTCCACGCCGAATATGACAAACTGACAGAATATTATCGGGGCCATAAAGAATGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16695.12 Da
• Isoelectric Point: 7.0634

>AT59964 WP_048214030.1 NZ_CP014030:c447529-447083 [Citrobacter sp. FDAARGOS_156]
MRSHRHMDAASAKKIVDTFSCAVKTIPLLGEERNETEYRRALALVEFLVDHDDLENPLFELLSARISEYEKHAPEFRALN
QHLKQIPPGVSVLRTLMDQYGLKATDLANELGSKSNVSNILNGRRALTVSHIKALAERFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT59964 NZ_CP014030:c447529-447083 [Citrobacter sp. FDAARGOS_156]
ATGAGATCTCATCGTCATATGGACGCAGCCAGCGCAAAAAAGATCGTTGATACCTTCAGTTGTGCTGTAAAAACGATCCC
TCTTTTGGGGGAAGAACGCAATGAAACGGAGTATCGAAGGGCACTAGCGCTAGTAGAGTTTCTGGTTGACCACGACGATC
TTGAAAACCCACTATTTGAATTACTTAGTGCCCGAATCAGTGAATACGAAAAACATGCACCAGAATTCAGAGCGCTCAAT
CAACACCTGAAACAAATTCCCCCTGGCGTTTCCGTATTGCGTACGCTCATGGATCAGTACGGATTAAAAGCCACCGACCT
CGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCCTAAATGGCCGTAGAGCACTGACTGTAAGCCATATTAAAG
CGCTTGCAGAACGCTTTAAACTTCCGGCAGATGCATTTATCGAGTAG