Detailed information of TA system TA059899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4372788..4373350
• Replicon: chromosome
• Accession: NZ_CP014015
• Organism: Citrobacter amalonaticus strain FDAARGOS_122

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3S7DBL2
• Locus tag: AL479_RS21125
• Protein ID: WP_043000694.1
• Coordinates: 4372788..4373066 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AL479_RS21130
• Protein ID: WP_061077524.1
• Coordinates: 4373066..4373350 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL479_RS21105	4367913..4368344	-	432	WP_061077520.1	peroxiredoxin OsmC	-
AL479_RS21110	4368572..4368715	+	144	WP_061077521.1	stationary-phase-induced ribosome-associated protein	-
AL479_RS21115	4368887..4370584	+	1698	WP_061077522.1	malate dehydrogenase	-
AL479_RS21120	4370893..4372608	+	1716	WP_061077523.1	ATP-binding cassette domain-containing protein	-
AL479_RS21125	4372788..4373066	+	279	WP_043000694.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AL479_RS21130	4373066..4373350	+	285	WP_061077524.1	HigA family addiction module antidote protein	Antitoxin
AL479_RS21135	4373395..4373997	-	603	WP_061077525.1	inorganic diphosphatase	-
AL479_RS21140	4374124..4374780	-	657	WP_061077526.1	formate dehydrogenase-N subunit gamma	-
AL479_RS21145	4374773..4375657	-	885	WP_061077527.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10573.12 Da
• Isoelectric Point: 8.9461

>T59899 WP_043000694.1 NZ_CP014015:4372788-4373066 [Citrobacter amalonaticus]
MIMNFRHKGLRDLFLHGKTSGVLAKHVKRLRHRLAVIDAAGCVNDINMPGYRLHPLSGDRDGVWSISVSGNWRMTFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T59899 NZ_CP014015:4372788-4373066 [Citrobacter amalonaticus]
ATGATCATGAATTTCAGGCACAAGGGATTACGCGACCTGTTTCTTCACGGTAAAACGTCAGGCGTGCTGGCAAAGCACGT
CAAACGTTTGCGACACCGCCTCGCCGTGATTGACGCCGCAGGCTGCGTGAACGATATCAACATGCCCGGTTACCGACTGC
ATCCCCTGAGCGGCGATCGTGATGGCGTCTGGTCAATATCGGTTTCAGGCAACTGGCGTATGACGTTCGAATTCGTCAAT
GGCGATGCGTACATTCTGGATTACGAGGATTATCACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10357.07 Da
• Isoelectric Point: 10.2982

>AT59899 WP_061077524.1 NZ_CP014015:4373066-4373350 [Citrobacter amalonaticus]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQSTWSLAQAQ
KSVDISRLRRLAVQ

Nucleotide

• Length: 285 bp

>AT59899 NZ_CP014015:4373066-4373350 [Citrobacter amalonaticus]
ATGAAAATGGCGAATCATCCCCGCCCCGGGGATATCATTCAGGAAGCGCTGGACGAACTGAACGTCAGTTTGCGTGAATT
TGCCAGAGCAATGGAGATTGCGCCCTCAACCGCCAGCCGGTTACTCACGGGCAAAGCGGCACTCACCCCGGAAATGGCGA
TCAAACTTTCGGTGGTGATCGGCAGTTCCCCGGAAATGTGGCTGAATCTGCAAAGTACCTGGAGTCTGGCGCAAGCGCAA
AAAAGCGTAGACATTTCCCGCCTGCGCCGTCTGGCGGTGCAGTAG

Structures

Toxin

• AlphaFold DB: A0A3S7DBL2