Detailed information of TA system TA059886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 184090..184817
• Replicon: plasmid pRJF999
• Accession: NZ_CP014011
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJF999

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: RJF9_RS27615
• Protein ID: WP_011251285.1
• Coordinates: 184506..184817 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RJF9_RS27610
• Protein ID: WP_011251286.1
• Coordinates: 184090..184509 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJF9_RS27580	179258..179728	-	471	WP_048333570.1	hypothetical protein	-
RJF9_RS31415	179921..180676	-	756	Protein_187	hypothetical protein	-
RJF9_RS27590	181272..181715	+	444	WP_048333569.1	hypothetical protein	-
RJF9_RS27595	181736..182524	+	789	WP_040217257.1	hypothetical protein	-
RJF9_RS27600	182538..182873	+	336	Protein_190	conjugal transfer protein TraI	-
RJF9_RS27605	182975..183943	-	969	WP_074428168.1	IS5 family transposase	-
RJF9_RS27610	184090..184509	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
RJF9_RS27615	184506..184817	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
RJF9_RS27620	185022..185480	-	459	Protein_194	DDE-type integrase/transposase/recombinase	-
RJF9_RS27630	185594..186291	+	698	Protein_195	IS1 family transposase	-
RJF9_RS27635	187677..188626	-	950	Protein_196	DUF1173 family protein	-
RJF9_RS27640	188804..189298	+	495	WP_004212794.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..228907	228907
	-	inside	IScluster/Tn	-	-	173969..186291	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T59886 WP_011251285.1 NZ_CP014011:c184817-184506 [Klebsiella pneumoniae subsp. pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T59886 NZ_CP014011:c184817-184506 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT59886 WP_011251286.1 NZ_CP014011:c184509-184090 [Klebsiella pneumoniae subsp. pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT59886 NZ_CP014011:c184509-184090 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1