Detailed information of TA system TA059866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 183638..184365
• Replicon: plasmid pRJF293
• Accession: NZ_CP014009
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJF293

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: RJF2_RS26465
• Protein ID: WP_011251285.1
• Coordinates: 183638..183949 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RJF2_RS26470
• Protein ID: WP_011251286.1
• Coordinates: 183946..184365 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJF2_RS26435	178676..180022	-	1347	WP_011154555.1	dihydroorotase	-
RJF2_RS26440	180019..180870	-	852	WP_004214538.1	M14 family metallocarboxypeptidase	-
RJF2_RS26445	180863..181864	-	1002	WP_011251272.1	TGS domain-containing protein	-
RJF2_RS26450	182023..182720	-	698	Protein_190	IS1 family transposase	-
RJF2_RS26460	182975..183433	+	459	Protein_191	DDE-type integrase/transposase/recombinase	-
RJF2_RS26465	183638..183949	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
RJF2_RS26470	183946..184365	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
RJF2_RS26475	184512..185480	+	969	WP_074428168.1	IS5 family transposase	-
RJF2_RS26480	185582..185917	-	336	Protein_195	conjugal transfer protein TraI	-
RJF2_RS26485	185931..186719	-	789	WP_040217257.1	hypothetical protein	-
RJF2_RS26490	186740..187183	-	444	WP_048333569.1	hypothetical protein	-
RJF2_RS29450	187779..188534	+	756	Protein_198	hypothetical protein	-
RJF2_RS26500	188727..189197	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2	1..224263	224263
	-	inside	IScluster/Tn	-	-	175797..194501	18704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T59866 WP_011251285.1 NZ_CP014009:183638-183949 [Klebsiella pneumoniae subsp. pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T59866 NZ_CP014009:183638-183949 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT59866 WP_011251286.1 NZ_CP014009:183946-184365 [Klebsiella pneumoniae subsp. pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT59866 NZ_CP014009:183946-184365 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1