Detailed information of TA system TA059830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1121467..1122116
• Replicon: chromosome
• Accession: NZ_CP014007
• Organism: Kosakonia oryzae strain Ola 51

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AWR26_RS05375
• Protein ID: WP_064564160.1
• Coordinates: 1121467..1121829 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A6M5DBJ4
• Locus tag: AWR26_RS05380
• Protein ID: WP_064564162.1
• Coordinates: 1121817..1122116 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWR26_RS05360	1117354..1118214	+	861	WP_064564154.1	aldo/keto reductase family oxidoreductase	-
AWR26_RS05365	1118318..1119313	-	996	WP_064564156.1	LacI family transcriptional regulator	-
AWR26_RS05370	1119335..1121200	-	1866	WP_064564158.1	beta-glucoside-specific PTS transporter subunit IIABC	-
AWR26_RS05375	1121467..1121829	+	363	WP_064564160.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AWR26_RS05380	1121817..1122116	+	300	WP_064564162.1	helix-turn-helix transcriptional regulator	Antitoxin
AWR26_RS05385	1122298..1122621	+	324	WP_064564164.1	antibiotic biosynthesis monooxygenase	-
AWR26_RS05390	1122614..1123003	+	390	WP_064564166.1	hypothetical protein	-
AWR26_RS05395	1123000..1123707	+	708	WP_064564168.1	winged helix-turn-helix domain-containing protein	-
AWR26_RS24785	1123761..1123898	+	138	Protein_1071	methylated-DNA--[protein]-cysteine S-methyltransferase	-
AWR26_RS05400	1123944..1125368	-	1425	WP_064564170.1	6-phospho-beta-glucosidase	-
AWR26_RS05405	1125389..1126831	-	1443	WP_064564172.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-84)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14061.25 Da
• Isoelectric Point: 8.9175

>T59830 WP_064564160.1 NZ_CP014007:1121467-1121829 [Kosakonia oryzae]
MWNVITTERFDEWFNKQTEPLQDEVLAILKILSEYGPHLGRPYVDTVKGSIFLNLKELRIQFAGHPVRAFFAFDPTRQAI
VLCAGDKTGKNQKRFYQDMIKLAETELSHHLKKKELTWQP

Nucleotide

• Length: 363 bp

>T59830 NZ_CP014007:1121467-1121829 [Kosakonia oryzae]
ATGTGGAACGTAATAACGACGGAGCGTTTTGATGAATGGTTTAATAAACAAACTGAACCTTTACAGGATGAAGTCCTGGC
GATCCTCAAAATATTGAGTGAATACGGTCCCCACCTCGGTCGGCCGTATGTGGATACGGTGAAGGGATCAATATTTTTGA
ATTTGAAGGAATTACGCATTCAATTTGCTGGCCATCCTGTTCGTGCATTTTTTGCTTTTGATCCAACCCGCCAGGCCATT
GTGTTATGCGCAGGCGATAAAACAGGAAAAAATCAGAAGCGGTTTTATCAAGATATGATCAAACTTGCTGAAACCGAGTT
AAGTCATCACCTGAAGAAAAAGGAGTTAACATGGCAACCTTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10814.56 Da
• Isoelectric Point: 8.6148

>AT59830 WP_064564162.1 NZ_CP014007:1121817-1122116 [Kosakonia oryzae]
MATLDEMLAKRSPESLARIESRVDELRRDIVLSQLREALNLSQTELAAAMGVKQPTLAKIEQPGNDPRLSTLKRYVAALG
GELSIDVTLPGGKKIAFQI

Nucleotide

• Length: 300 bp

>AT59830 NZ_CP014007:1121817-1122116 [Kosakonia oryzae]
ATGGCAACCTTAGATGAGATGCTAGCCAAACGAAGCCCGGAGAGCCTTGCGCGCATTGAATCTCGTGTCGATGAACTTCG
CCGCGATATCGTATTAAGTCAGTTACGGGAAGCATTGAATCTTTCTCAAACCGAACTGGCAGCGGCAATGGGCGTGAAGC
AACCTACGCTGGCCAAAATTGAGCAACCGGGCAATGATCCGCGCTTATCGACGCTTAAACGTTATGTCGCGGCCTTGGGC
GGCGAATTGAGCATTGATGTCACTTTGCCGGGCGGTAAAAAGATCGCGTTTCAAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6M5DBJ4