Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2249939..2250536 Replicon chromosome
Accession NZ_CP014004
Organism Klebsiella pneumoniae subsp. pneumoniae strain NUHL24835

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag AWN66_RS11015 Protein ID WP_004142563.1
Coordinates 2250219..2250536 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag AWN66_RS11010 Protein ID WP_004142561.1
Coordinates 2249939..2250226 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWN66_RS10980 2246019..2246267 + 249 WP_002893037.1 DUF1158 domain-containing protein -
AWN66_RS10985 2246285..2246626 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
AWN66_RS10990 2246657..2247772 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
AWN66_RS10995 2247952..2248533 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
AWN66_RS11000 2248533..2248901 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
AWN66_RS11005 2249021..2249674 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
AWN66_RS11010 2249939..2250226 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
AWN66_RS11015 2250219..2250536 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AWN66_RS11020 2250721..2251764 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
AWN66_RS11025 2252431..2253297 - 867 WP_059689649.1 helix-turn-helix transcriptional regulator -
AWN66_RS11030 2253406..2254833 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T59819 WP_004142563.1 NZ_CP014004:c2250536-2250219 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T59819 NZ_CP014004:c2250536-2250219 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT59819 WP_004142561.1 NZ_CP014004:c2250226-2249939 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT59819 NZ_CP014004:c2250226-2249939 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References