Detailed information of TA system TA059808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 24823..25431
• Replicon: plasmid unnamed3
• Accession: NZ_CP014001
• Organism: Synechococcus sp. PCC 73109

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: AWQ23_RS14595
• Protein ID: WP_062436823.1
• Coordinates: 25150..25431 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AWQ23_RS14590
• Protein ID: WP_062436820.1
• Coordinates: 24823..25137 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWQ23_RS14565	20420..20647	-	228	WP_062436816.1	hypothetical protein	-
AWQ23_RS14570	20647..21081	-	435	WP_062436854.1	PIN domain-containing protein	-
AWQ23_RS14575	21095..22879	-	1785	WP_062436855.1	ATP-binding protein	-
AWQ23_RS14580	22901..24115	-	1215	WP_062436818.1	DNA double-strand break repair nuclease NurA	-
AWQ23_RS14585	24175..24501	+	327	Protein_22	nucleotide pyrophosphohydrolase	-
AWQ23_RS14590	24823..25137	-	315	WP_062436820.1	HigA family addiction module antidote protein	Antitoxin
AWQ23_RS14595	25150..25431	-	282	WP_062436823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AWQ23_RS16245	25574..25795	-	222	WP_062436825.1	hypothetical protein	-
AWQ23_RS14605	25818..27683	-	1866	WP_083500755.1	AAA family ATPase	-
AWQ23_RS14620	28379..30205	-	1827	WP_062436829.1	TM0106 family RecB-like putative nuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38214	38214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11187.81 Da
• Isoelectric Point: 10.7019

>T59808 WP_062436823.1 NZ_CP014001:c25431-25150 [Synechococcus sp. PCC 73109]
VIRSFKDKETQKIFERQHSRKLPSEIQQVVLRKLRMLNRAETLQDLRVPPANRLEALRGSRQGQYSIRINNQWRICFQWE
NGEALNVEIVDYH

Nucleotide

• Length: 282 bp

>T59808 NZ_CP014001:c25431-25150 [Synechococcus sp. PCC 73109]
GTGATTCGCAGTTTCAAAGATAAAGAAACCCAAAAAATTTTTGAACGACAGCATTCCCGTAAACTTCCTAGCGAAATTCA
ACAGGTTGTCCTGAGGAAATTACGAATGCTTAATCGGGCAGAAACTCTCCAAGATCTGAGAGTACCGCCAGCAAACCGCT
TAGAAGCCCTGAGGGGCTCCCGCCAGGGCCAATACAGTATTCGCATCAATAACCAATGGCGTATTTGTTTCCAGTGGGAG
AATGGCGAAGCCCTTAATGTAGAAATTGTTGACTATCACTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11922.88 Da
• Isoelectric Point: 4.8605

>AT59808 WP_062436820.1 NZ_CP014001:c25137-24823 [Synechococcus sp. PCC 73109]
MTQEKLLPIHPGEVLLEEFLKPMNLSQNKLALAIRVPARRINEIVQGKRRITADTALRLGLYFNMSPQFWLGLQMDYDLD
IAEDEFGEKLQREVIPAELISTTA

Nucleotide

• Length: 315 bp

>AT59808 NZ_CP014001:c25137-24823 [Synechococcus sp. PCC 73109]
ATGACACAAGAGAAGCTATTACCCATCCATCCCGGCGAAGTTCTGCTCGAAGAATTTCTCAAACCAATGAACCTCAGTCA
GAACAAGCTTGCCCTAGCGATTCGTGTACCCGCCCGTCGGATTAACGAAATTGTTCAGGGCAAACGTCGTATAACTGCTG
ATACAGCTTTGCGCCTAGGCCTTTATTTCAATATGTCTCCTCAATTTTGGCTTGGTTTACAGATGGACTATGACCTAGAT
ATTGCAGAAGATGAGTTTGGTGAAAAACTGCAACGGGAGGTGATCCCTGCAGAGCTCATCTCTACTACTGCCTAA