Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 373440..373975 Replicon plasmid unnamed1
Accession NZ_CP013995
Organism Enterococcus faecium strain 6E6

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag AWJ25_RS16685 Protein ID WP_002314366.1
Coordinates 373703..373975 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag AWJ25_RS16680 Protein ID WP_002297422.1
Coordinates 373440..373703 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWJ25_RS16665 368868..371117 + 2250 WP_002314387.1 alpha-galactosidase -
AWJ25_RS16670 371120..372295 + 1176 WP_002336205.1 MFS transporter -
AWJ25_RS16675 372298..373206 - 909 Protein_406 IS982 family transposase -
AWJ25_RS16680 373440..373703 + 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AWJ25_RS16685 373703..373975 + 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
AWJ25_RS16690 374052..374381 + 330 WP_002292678.1 AbrB family transcriptional regulator -
AWJ25_RS16695 374371..374657 + 287 Protein_410 type II toxin-antitoxin system RelE/ParE family toxin -
AWJ25_RS16700 374943..375800 + 858 WP_002292680.1 AAA family ATPase -
AWJ25_RS18335 375801..375941 + 141 WP_154591461.1 hypothetical protein -
AWJ25_RS16705 375975..376655 - 681 WP_002331383.1 IS6-like element IS1216 family transposase -
AWJ25_RS18170 376731..377374 - 644 Protein_414 transposase -
AWJ25_RS18635 377649..378191 + 543 WP_002298088.1 hypothetical protein -
AWJ25_RS16725 378347..378796 + 450 WP_010729386.1 TlpA family protein disulfide reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..381524 381524
- inside IScluster/Tn - - 360948..379988 19040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T59804 WP_002314366.1 NZ_CP013995:373703-373975 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T59804 NZ_CP013995:373703-373975 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT59804 WP_002297422.1 NZ_CP013995:373440-373703 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT59804 NZ_CP013995:373440-373703 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References