Detailed information of TA system TA059775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5354658..5355253
• Replicon: chromosome
• Accession: NZ_CP013989
• Organism: Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: APT60_RS30160
• Protein ID: WP_003113526.1
• Coordinates: 5354975..5355253 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: APT60_RS24625
• Protein ID: WP_003099268.1
• Coordinates: 5354658..5354963 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APT60_RS24590	5349808..5350656	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
APT60_RS24600	5350823..5351764	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
APT60_RS24605	5351881..5352495	+	615	WP_059349764.1	50S ribosomal protein L25/general stress protein Ctc	-
APT60_RS24610	5352537..5353121	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
APT60_RS24615	5353162..5354262	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
APT60_RS24625	5354658..5354963	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
APT60_RS30160	5354975..5355253	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
APT60_RS24630	5355582..5357810	+	2229	WP_003099265.1	TonB-dependent receptor	-
APT60_RS24635	5357880..5358527	-	648	WP_003095021.1	carbonate dehydratase	-
APT60_RS24640	5358589..5359827	-	1239	WP_059349765.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T59775 WP_003113526.1 NZ_CP013989:c5355253-5354975 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T59775 NZ_CP013989:c5355253-5354975 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT59775 WP_003099268.1 NZ_CP013989:c5354963-5354658 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT59775 NZ_CP013989:c5354963-5354658 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3