Detailed information of TA system TA059760

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4175724..4176321
• Replicon: chromosome
• Accession: NZ_CP013985
• Organism: Klebsiella variicola strain LMG 23571

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: AWN63_RS20775
• Protein ID: WP_012542526.1
• Coordinates: 4176004..4176321 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AWN63_RS20770
• Protein ID: WP_012542525.1
• Coordinates: 4175724..4176011 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWN63_RS20740	4171584..4171832	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
AWN63_RS20745	4171849..4172190	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
AWN63_RS20750	4172221..4173336	-	1116	WP_162499993.1	MBL fold metallo-hydrolase	-
AWN63_RS20755	4173516..4174097	+	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
AWN63_RS20760	4174097..4174465	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
AWN63_RS20765	4174585..4175238	+	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
AWN63_RS20770	4175724..4176011	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
AWN63_RS20775	4176004..4176321	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AWN63_RS20780	4176506..4177549	-	1044	WP_119867102.1	DUF2157 domain-containing protein	-
AWN63_RS20790	4178210..4179076	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
AWN63_RS20795	4179185..4180612	+	1428	WP_049009040.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T59760 WP_012542526.1 NZ_CP013985:c4176321-4176004 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T59760 NZ_CP013985:c4176321-4176004 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGTTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCATCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT59760 WP_012542525.1 NZ_CP013985:c4176011-4175724 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT59760 NZ_CP013985:c4176011-4175724 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2