Detailed information of TA system TA059742

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): holin-SprF1/-
• Location: 943826..944196
• Replicon: chromosome
• Accession: NZ_CP013980
• Organism: Staphylococcus equorum strain KM1031

Toxin (Protein)

• Gene name: holin
• Uniprot ID: -
• Locus tag: AWC34_RS04515
• Protein ID: WP_064783025.1
• Coordinates: 943975..944196 (+)
• Length: 74 a.a.

Antitoxin (RNA)

Gene name	SprF1
Locus tag	-
Coordinates	943826..943924 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWC34_RS04490	940138..940959	+	822	WP_002507957.1	succinate dehydrogenase iron-sulfur subunit	-
AWC34_RS04495	941235..942029	+	795	WP_002507956.1	glutamate racemase	-
AWC34_RS04500	942047..942628	+	582	WP_002507955.1	XTP/dITP diphosphatase	-
AWC34_RS04505	942628..943137	+	510	WP_002507954.1	metallophosphoesterase	-
-	943826..943924	-	99	-	-	Antitoxin
AWC34_RS04515	943975..944196	+	222	WP_064783025.1	phage holin	Toxin
AWC34_RS13355	944351..944467	+	117	WP_165349553.1	putative holin-like toxin	-
AWC34_RS13360	944766..944873	+	108	WP_021338681.1	putative holin-like toxin	-
AWC34_RS04520	945309..945725	+	417	WP_081288848.1	hypothetical protein	-
AWC34_RS04525	945709..946017	+	309	WP_081288849.1	hypothetical protein	-
AWC34_RS04530	946324..948207	+	1884	WP_046465189.1	glycosyltransferase family 2 protein	-
AWC34_RS04535	948269..948826	-	558	WP_046465190.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Holin (7-57)

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8134.67 Da
• Isoelectric Point: 6.1486

>T59742 WP_064783025.1 NZ_CP013980:943975-944196 [Staphylococcus equorum]
MDVKVTVIVRVLSLILVLVNQWLSNKGVSPIPVDESTLMTIAVALITMLKDNPFTDAAKGSFFKSYTYQLEIF

Nucleotide

• Length: 222 bp

>T59742 NZ_CP013980:943975-944196 [Staphylococcus equorum]
ATGGATGTAAAAGTAACAGTAATCGTAAGAGTATTAAGTTTAATTTTGGTGTTAGTGAACCAGTGGTTGTCAAATAAAGG
AGTGAGTCCTATTCCAGTGGATGAGTCAACACTAATGACTATCGCAGTCGCATTAATCACGATGTTGAAAGATAACCCAT
TTACAGATGCAGCTAAGGGTTCTTTTTTTAAATCTTATACTTATCAGCTTGAAATATTTTGA

Antitoxin

Nucleotide

• Length: 99 bp

>AT59742 NZ_CP013980:c943924-943826 [Staphylococcus equorum]
ATTTGATAATATTTATATGTTGTGTTAATTTATATATAGAAAAAGGGCAACACACCATAAGTGTATCGCCCCAATGAGCC
CGTTAAAAAGACGGTGGCT

Structures

Antitoxin