Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 26331..26867 Replicon plasmid pPSCRG02-4
Accession NZ_CP013978
Organism Piscirickettsia salmonis strain CGR02

Toxin (Protein)


Gene name yoeB Uniprot ID A0A6I5YBD1
Locus tag AWJ11_RS16970 Protein ID WP_016211910.1
Coordinates 26331..26603 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A0B8U9U2
Locus tag AWJ11_RS16975 Protein ID WP_032126795.1
Coordinates 26607..26867 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWJ11_RS20035 22260..22517 - 258 WP_098082828.1 transposase -
AWJ11_RS18780 22750..23667 + 918 Protein_24 IS30 family transposase -
AWJ11_RS16950 23784..24248 + 465 WP_036774631.1 hypothetical protein -
AWJ11_RS18785 24307..24447 - 141 Protein_26 helix-turn-helix domain-containing protein -
AWJ11_RS18790 24611..24757 + 147 WP_157894761.1 transposase zinc-binding domain-containing protein -
AWJ11_RS16960 25474..25614 - 141 Protein_28 helix-turn-helix domain-containing protein -
AWJ11_RS16965 25787..26005 + 219 WP_155046644.1 hypothetical protein -
AWJ11_RS16970 26331..26603 - 273 WP_016211910.1 Txe/YoeB family addiction module toxin Toxin
AWJ11_RS16975 26607..26867 - 261 WP_032126795.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AWJ11_RS16980 27140..27730 + 591 WP_027242600.1 site-specific integrase -
AWJ11_RS18795 27758..29161 - 1404 WP_048876031.1 IS4 family transposase -
AWJ11_RS18800 29865..30710 - 846 Protein_34 IS982 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..68882 68882
- flank IS/Tn - - 30193..30387 194


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-74)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10739.22 Da        Isoelectric Point: 7.8926

>T59740 WP_016211910.1 NZ_CP013978:c26603-26331 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRTPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
IAACRYHYDQ

Download         Length: 273 bp

>T59740 NZ_CP013978:c26603-26331 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
ACTTAAAAAAATCAATAAACTTATAAAGGAATGTCAACGCACGCCGACAGAAGGTATTGGTCATCCCGAACCTTTAAAAC
ATGATTTCAGTGGCTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTGCTGCATGCCGTTACCATTATGATCAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9555.93 Da        Isoelectric Point: 5.1167

>AT59740 WP_032126795.1 NZ_CP013978:c26867-26607 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Download         Length: 261 bp

>AT59740 NZ_CP013978:c26867-26607 [Piscirickettsia salmonis]
ATGTTACGAGACAACATTAATGCTACGGCAGCGCGGGATAATTTTAAGAGTACCTGTGATACGGTGTGTGAAAACAATGC
CCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGCATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I5YBD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0B8U9U2

References