Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 7334..7870 Replicon plasmid pPSCRG02-1
Accession NZ_CP013976
Organism Piscirickettsia salmonis strain CGR02

Toxin (Protein)


Gene name yoeB Uniprot ID A0A1L6THY5
Locus tag AWJ11_RS15950 Protein ID WP_036772441.1
Coordinates 7598..7870 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A0B8U9U2
Locus tag AWJ11_RS15945 Protein ID WP_032126795.1
Coordinates 7334..7594 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWJ11_RS15910 3109..4236 + 1128 WP_036772450.1 hypothetical protein -
AWJ11_RS18645 4316..4579 + 264 Protein_5 IS6 family transposase -
AWJ11_RS15920 4968..5366 + 399 Protein_6 IS6 family transposase -
AWJ11_RS15930 5690..5947 - 258 Protein_7 DDE-type integrase/transposase/recombinase -
AWJ11_RS15935 6236..6544 - 309 Protein_8 IS6 family transposase -
AWJ11_RS19915 6830..6994 - 165 Protein_9 IS6 family transposase -
AWJ11_RS15945 7334..7594 + 261 WP_032126795.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AWJ11_RS15950 7598..7870 + 273 WP_036772441.1 Txe/YoeB family addiction module toxin Toxin
AWJ11_RS15955 7946..8674 + 729 WP_017375910.1 IS6 family transposase -
AWJ11_RS15960 9243..10307 - 1065 WP_174872554.1 IS91 family transposase -
AWJ11_RS20630 10487..10666 - 180 WP_157894752.1 hypothetical protein -
AWJ11_RS15970 11079..11906 + 828 WP_048876208.1 IS30 family transposase -
AWJ11_RS15975 11949..12678 - 730 Protein_16 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..115926 115926
- inside IScluster/Tn - - 4316..18292 13976


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(7-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-74)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10785.31 Da        Isoelectric Point: 7.8926

>T59736 WP_036772441.1 NZ_CP013976:7598-7870 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRMPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
ISACRYHYDQ

Download         Length: 273 bp

>T59736 NZ_CP013976:7598-7870 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
GCTTAAAAAAATCAATAAACTTATAAAGGAATGTCAACGCATGCCGACAGAAGGTATTGGTCATCCCGAACCTTTAAAAC
ATGATTTCAGTGGCTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTTCTGCATGCCGTTACCATTATGATCAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9555.93 Da        Isoelectric Point: 5.1167

>AT59736 WP_032126795.1 NZ_CP013976:7334-7594 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Download         Length: 261 bp

>AT59736 NZ_CP013976:7334-7594 [Piscirickettsia salmonis]
ATGCTACGAGATAACATCAATGCTACGGCAGCACGGGATAATTTTAAGAGTACCTGTGATACAGTGTGTGAAAACAATGC
TCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGTATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L6THY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B8U9U2

References